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Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

Genome-edited human pluripotent stem cells (hPSCs) have broad applications in disease modeling, drug discovery, and regenerative medicine. We present and characterize a robust method for rapid, scarless introduction or correction of disease-associated variants in hPSCs using CRISPR/Cas9. Utilizing n...

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Detalles Bibliográficos
Autores principales:	Steyer, Benjamin, Bu, Qian, Cory, Evan, Jiang, Keer, Duong, Stella, Sinha, Divya, Steltzer, Stephanie, Gamm, David, Chang, Qiang, Saha, Krishanu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2018
Materias:	Resource
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830934/ https://www.ncbi.nlm.nih.gov/pubmed/29307579 http://dx.doi.org/10.1016/j.stemcr.2017.12.004

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