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Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction
Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardia...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830958/ https://www.ncbi.nlm.nih.gov/pubmed/29398480 http://dx.doi.org/10.1016/j.stemcr.2018.01.002 |
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author | Zhao, Xin Chen, Haodong Xiao, Dan Yang, Huaxiao Itzhaki, Ilanit Qin, Xulei Chour, Tony Aguirre, Aitor Lehmann, Kim Kim, Youngkyun Shukla, Praveen Holmström, Alexandra Zhang, Joe Z. Zhuge, Yan Ndoye, Babacar C. Zhao, Mingtao Neofytou, Evgenios Zimmermann, Wolfram-Hubertus Jain, Mohit Wu, Joseph C. |
author_facet | Zhao, Xin Chen, Haodong Xiao, Dan Yang, Huaxiao Itzhaki, Ilanit Qin, Xulei Chour, Tony Aguirre, Aitor Lehmann, Kim Kim, Youngkyun Shukla, Praveen Holmström, Alexandra Zhang, Joe Z. Zhuge, Yan Ndoye, Babacar C. Zhao, Mingtao Neofytou, Evgenios Zimmermann, Wolfram-Hubertus Jain, Mohit Wu, Joseph C. |
author_sort | Zhao, Xin |
collection | PubMed |
description | Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × 10(7) NHP or human iPSC-CMs or the same number of respective fibroblasts or PBS control (n = 9–14/group) at 4 days after 60-min coronary artery occlusion-reperfusion. Cardiac function and left ventricular remodeling were similarly improved in both iPSC-CM-treated groups. To mimic the ischemic environment in the infarcted heart, both cultured NHP and human iPSC-CMs underwent 24-hr hypoxia in vitro. Both cells and media were collected, and similarities in transcriptomic as well as metabolomic profiles were noted between both groups. In conclusion, both NHP and human iPSC-CMs confer similar cardioprotection in a rodent myocardial infarction model through relatively similar mechanisms via promotion of cell survival, angiogenesis, and inhibition of hypertrophy and fibrosis. |
format | Online Article Text |
id | pubmed-5830958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-58309582018-03-06 Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction Zhao, Xin Chen, Haodong Xiao, Dan Yang, Huaxiao Itzhaki, Ilanit Qin, Xulei Chour, Tony Aguirre, Aitor Lehmann, Kim Kim, Youngkyun Shukla, Praveen Holmström, Alexandra Zhang, Joe Z. Zhuge, Yan Ndoye, Babacar C. Zhao, Mingtao Neofytou, Evgenios Zimmermann, Wolfram-Hubertus Jain, Mohit Wu, Joseph C. Stem Cell Reports Article Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × 10(7) NHP or human iPSC-CMs or the same number of respective fibroblasts or PBS control (n = 9–14/group) at 4 days after 60-min coronary artery occlusion-reperfusion. Cardiac function and left ventricular remodeling were similarly improved in both iPSC-CM-treated groups. To mimic the ischemic environment in the infarcted heart, both cultured NHP and human iPSC-CMs underwent 24-hr hypoxia in vitro. Both cells and media were collected, and similarities in transcriptomic as well as metabolomic profiles were noted between both groups. In conclusion, both NHP and human iPSC-CMs confer similar cardioprotection in a rodent myocardial infarction model through relatively similar mechanisms via promotion of cell survival, angiogenesis, and inhibition of hypertrophy and fibrosis. Elsevier 2018-02-01 /pmc/articles/PMC5830958/ /pubmed/29398480 http://dx.doi.org/10.1016/j.stemcr.2018.01.002 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Xin Chen, Haodong Xiao, Dan Yang, Huaxiao Itzhaki, Ilanit Qin, Xulei Chour, Tony Aguirre, Aitor Lehmann, Kim Kim, Youngkyun Shukla, Praveen Holmström, Alexandra Zhang, Joe Z. Zhuge, Yan Ndoye, Babacar C. Zhao, Mingtao Neofytou, Evgenios Zimmermann, Wolfram-Hubertus Jain, Mohit Wu, Joseph C. Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title | Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title_full | Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title_fullStr | Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title_full_unstemmed | Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title_short | Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction |
title_sort | comparison of non-human primate versus human induced pluripotent stem cell-derived cardiomyocytes for treatment of myocardial infarction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830958/ https://www.ncbi.nlm.nih.gov/pubmed/29398480 http://dx.doi.org/10.1016/j.stemcr.2018.01.002 |
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