Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells

Default differentiation of human pluripotent stem cells has been promoted as a model of cortical development. In this study, a developmental transcriptome analysis of default-differentiated hPSNs revealed a gene expression program resembling in vivo CGE/LGE subpallial domains and GABAergic signaling...

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Autores principales: Floruta, Crina M., Du, Ruofei, Kang, Huining, Stein, Jason L., Weick, Jason P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831028/
https://www.ncbi.nlm.nih.gov/pubmed/29107596
http://dx.doi.org/10.1016/j.stemcr.2017.09.023
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author Floruta, Crina M.
Du, Ruofei
Kang, Huining
Stein, Jason L.
Weick, Jason P.
author_facet Floruta, Crina M.
Du, Ruofei
Kang, Huining
Stein, Jason L.
Weick, Jason P.
author_sort Floruta, Crina M.
collection PubMed
description Default differentiation of human pluripotent stem cells has been promoted as a model of cortical development. In this study, a developmental transcriptome analysis of default-differentiated hPSNs revealed a gene expression program resembling in vivo CGE/LGE subpallial domains and GABAergic signaling. A combination of bioinformatic, functional, and immunocytochemical analysis further revealed that hPSNs consist of both cortical glutamatergic and CGE-like GABAergic neurons. This study provides a comprehensive characterization of the heterogeneous group of neurons produced by default differentiation and insight into future directed differentiation strategies.
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spelling pubmed-58310282018-03-06 Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells Floruta, Crina M. Du, Ruofei Kang, Huining Stein, Jason L. Weick, Jason P. Stem Cell Reports Article Default differentiation of human pluripotent stem cells has been promoted as a model of cortical development. In this study, a developmental transcriptome analysis of default-differentiated hPSNs revealed a gene expression program resembling in vivo CGE/LGE subpallial domains and GABAergic signaling. A combination of bioinformatic, functional, and immunocytochemical analysis further revealed that hPSNs consist of both cortical glutamatergic and CGE-like GABAergic neurons. This study provides a comprehensive characterization of the heterogeneous group of neurons produced by default differentiation and insight into future directed differentiation strategies. Elsevier 2017-10-26 /pmc/articles/PMC5831028/ /pubmed/29107596 http://dx.doi.org/10.1016/j.stemcr.2017.09.023 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Floruta, Crina M.
Du, Ruofei
Kang, Huining
Stein, Jason L.
Weick, Jason P.
Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title_full Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title_fullStr Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title_full_unstemmed Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title_short Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells
title_sort default patterning produces pan-cortical glutamatergic and cge/lge-like gabaergic neurons from human pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831028/
https://www.ncbi.nlm.nih.gov/pubmed/29107596
http://dx.doi.org/10.1016/j.stemcr.2017.09.023
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