Cargando…

Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension

BACKGROUND: Hypertension is a risk factor for both cardiovascular diseases (CVDs) and type 2 diabetes (T2D). Angiopoietin-like proteins (ANGPTLs), mainly ANGPTL3, ANGPTL4 and ANGPTL8, are associated with increased plasma lipid content due to their role in regulating the activity of lipoprotein lipas...

Descripción completa

Detalles Bibliográficos
Autores principales: Abu-Farha, Mohamed, Cherian, Preethi, Qaddoumi, Mohamed G., AlKhairi, Irina, Sriraman, Devarajan, Alanbaei, Muath, Abubaker, Jehad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831738/
https://www.ncbi.nlm.nih.gov/pubmed/29490644
http://dx.doi.org/10.1186/s12944-018-0681-0
_version_ 1783303190909812736
author Abu-Farha, Mohamed
Cherian, Preethi
Qaddoumi, Mohamed G.
AlKhairi, Irina
Sriraman, Devarajan
Alanbaei, Muath
Abubaker, Jehad
author_facet Abu-Farha, Mohamed
Cherian, Preethi
Qaddoumi, Mohamed G.
AlKhairi, Irina
Sriraman, Devarajan
Alanbaei, Muath
Abubaker, Jehad
author_sort Abu-Farha, Mohamed
collection PubMed
description BACKGROUND: Hypertension is a risk factor for both cardiovascular diseases (CVDs) and type 2 diabetes (T2D). Angiopoietin-like proteins (ANGPTLs), mainly ANGPTL3, ANGPTL4 and ANGPTL8, are associated with increased plasma lipid content due to their role in regulating the activity of lipoprotein lipase, a key enzyme in metabolism of the lipoprotein in circulation. Dyslipidaemia is a risk factor for hypertension development; however, the roles of ANGPTL3, ANGPTL4 and ANGPTL8 in subjects with hypertension have not yet been established. This study compared the plasma and adipose tissue levels of ANGPTL3, ANGPTL4 and ANGPTL8 in age- and body mass index-matched subjects with and without hypertension. METHODS: A total of 119 subjects, including 69 hypertensive and 50 non-hypertensive subjects, were enrolled. ANGPTL3, ANGPTL4 and ANGPTL8 plasma levels were measured by ELISA, whereas their levels in adipose tissue were assessed via real-time PCR. RESULTS: We found that ANGPTL4 (202.49 ± 17.44 ng/mL vs. 160.64 ± 10.36 ng/mL, p = 0.04) and ANGPTL8 levels (2310.96 ± 194.88 pg/mL vs. 1583.35 ± 138.27 pg/mL, p = 0.001) were higher in hypertensive subjects than non-hypertensive subjects. However, ANGPTL3 levels were not significantly different between the two populations. Similarly, ANGPTL4 and ANGPTL8 levels were also elevated in subjects with T2D and hypertension than in those with T2D but not hypertension. Additionally, people with highest tertiles of ANGPTL8 had higher odds of having hypertension (odd ratio [OR] = 3.8, 95% confidence interval [CI] = (1.5-9.8), p-Value = 0.005. Similar to its plasma levels, ANGPTL4 and ANGPTL8 were higher in adipose tissue. CONCLUSIONS: In conclusion, our data illustrate that ANGPTL4 and ANGPTL8 levels in both plasma and adipose tissues are increased in subjects with hypertension. The elevated levels of ANGPTL4 and ANGPTL8 in hypertensive subjects highlight their potential involvement, their potential role as biomarkers for hypertension and their therapeutic value in hypertension given their roles in regulating lipid metabolism.
format Online
Article
Text
id pubmed-5831738
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58317382018-03-05 Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension Abu-Farha, Mohamed Cherian, Preethi Qaddoumi, Mohamed G. AlKhairi, Irina Sriraman, Devarajan Alanbaei, Muath Abubaker, Jehad Lipids Health Dis Research BACKGROUND: Hypertension is a risk factor for both cardiovascular diseases (CVDs) and type 2 diabetes (T2D). Angiopoietin-like proteins (ANGPTLs), mainly ANGPTL3, ANGPTL4 and ANGPTL8, are associated with increased plasma lipid content due to their role in regulating the activity of lipoprotein lipase, a key enzyme in metabolism of the lipoprotein in circulation. Dyslipidaemia is a risk factor for hypertension development; however, the roles of ANGPTL3, ANGPTL4 and ANGPTL8 in subjects with hypertension have not yet been established. This study compared the plasma and adipose tissue levels of ANGPTL3, ANGPTL4 and ANGPTL8 in age- and body mass index-matched subjects with and without hypertension. METHODS: A total of 119 subjects, including 69 hypertensive and 50 non-hypertensive subjects, were enrolled. ANGPTL3, ANGPTL4 and ANGPTL8 plasma levels were measured by ELISA, whereas their levels in adipose tissue were assessed via real-time PCR. RESULTS: We found that ANGPTL4 (202.49 ± 17.44 ng/mL vs. 160.64 ± 10.36 ng/mL, p = 0.04) and ANGPTL8 levels (2310.96 ± 194.88 pg/mL vs. 1583.35 ± 138.27 pg/mL, p = 0.001) were higher in hypertensive subjects than non-hypertensive subjects. However, ANGPTL3 levels were not significantly different between the two populations. Similarly, ANGPTL4 and ANGPTL8 levels were also elevated in subjects with T2D and hypertension than in those with T2D but not hypertension. Additionally, people with highest tertiles of ANGPTL8 had higher odds of having hypertension (odd ratio [OR] = 3.8, 95% confidence interval [CI] = (1.5-9.8), p-Value = 0.005. Similar to its plasma levels, ANGPTL4 and ANGPTL8 were higher in adipose tissue. CONCLUSIONS: In conclusion, our data illustrate that ANGPTL4 and ANGPTL8 levels in both plasma and adipose tissues are increased in subjects with hypertension. The elevated levels of ANGPTL4 and ANGPTL8 in hypertensive subjects highlight their potential involvement, their potential role as biomarkers for hypertension and their therapeutic value in hypertension given their roles in regulating lipid metabolism. BioMed Central 2018-03-01 /pmc/articles/PMC5831738/ /pubmed/29490644 http://dx.doi.org/10.1186/s12944-018-0681-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Abu-Farha, Mohamed
Cherian, Preethi
Qaddoumi, Mohamed G.
AlKhairi, Irina
Sriraman, Devarajan
Alanbaei, Muath
Abubaker, Jehad
Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title_full Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title_fullStr Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title_full_unstemmed Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title_short Increased plasma and adipose tissue levels of ANGPTL8/Betatrophin and ANGPTL4 in people with hypertension
title_sort increased plasma and adipose tissue levels of angptl8/betatrophin and angptl4 in people with hypertension
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831738/
https://www.ncbi.nlm.nih.gov/pubmed/29490644
http://dx.doi.org/10.1186/s12944-018-0681-0
work_keys_str_mv AT abufarhamohamed increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT cherianpreethi increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT qaddoumimohamedg increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT alkhairiirina increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT sriramandevarajan increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT alanbaeimuath increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension
AT abubakerjehad increasedplasmaandadiposetissuelevelsofangptl8betatrophinandangptl4inpeoplewithhypertension