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Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis
Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831811/ https://www.ncbi.nlm.nih.gov/pubmed/29615575 http://dx.doi.org/10.3390/jdb4010006 |
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author | Gening, Leonid V. Lakhin, Andrei V. Makarova, Irina V. Nenasheva, Valentina V. Andreeva, Ludmila E. Tarantul, Vyacheslav Z. |
author_facet | Gening, Leonid V. Lakhin, Andrei V. Makarova, Irina V. Nenasheva, Valentina V. Andreeva, Ludmila E. Tarantul, Vyacheslav Z. |
author_sort | Gening, Leonid V. |
collection | PubMed |
description | Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study. |
format | Online Article Text |
id | pubmed-5831811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58318112018-03-30 Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis Gening, Leonid V. Lakhin, Andrei V. Makarova, Irina V. Nenasheva, Valentina V. Andreeva, Ludmila E. Tarantul, Vyacheslav Z. J Dev Biol Article Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study. MDPI 2016-01-27 /pmc/articles/PMC5831811/ /pubmed/29615575 http://dx.doi.org/10.3390/jdb4010006 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gening, Leonid V. Lakhin, Andrei V. Makarova, Irina V. Nenasheva, Valentina V. Andreeva, Ludmila E. Tarantul, Vyacheslav Z. Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title | Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title_full | Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title_fullStr | Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title_full_unstemmed | Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title_short | Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis |
title_sort | alterations in synthesis and repair of dna during the development of loach misgurnus fossilis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831811/ https://www.ncbi.nlm.nih.gov/pubmed/29615575 http://dx.doi.org/10.3390/jdb4010006 |
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