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The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: grou...

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Autores principales: Prakoso, Yos Adi, Kurniasih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832127/
https://www.ncbi.nlm.nih.gov/pubmed/29666657
http://dx.doi.org/10.1155/2018/6218303
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author Prakoso, Yos Adi
Kurniasih,
author_facet Prakoso, Yos Adi
Kurniasih,
author_sort Prakoso, Yos Adi
collection PubMed
description The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4(+) and CD8(+) lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8(+) lymphocytes and increased the epidermal thickness and the expression of CD4(+) lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4(+)/CD8(+) lymphocytes in the wound area.
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spelling pubmed-58321272018-04-17 The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing Prakoso, Yos Adi Kurniasih, J Trop Med Research Article The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4(+) and CD8(+) lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8(+) lymphocytes and increased the epidermal thickness and the expression of CD4(+) lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4(+)/CD8(+) lymphocytes in the wound area. Hindawi 2018-02-15 /pmc/articles/PMC5832127/ /pubmed/29666657 http://dx.doi.org/10.1155/2018/6218303 Text en Copyright © 2018 Yos Adi Prakoso and Kurniasih. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Prakoso, Yos Adi
Kurniasih,
The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title_full The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title_fullStr The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title_full_unstemmed The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title_short The Effects of Aloe vera Cream on the Expression of CD4(+) and CD8(+) Lymphocytes in Skin Wound Healing
title_sort effects of aloe vera cream on the expression of cd4(+) and cd8(+) lymphocytes in skin wound healing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832127/
https://www.ncbi.nlm.nih.gov/pubmed/29666657
http://dx.doi.org/10.1155/2018/6218303
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