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Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients

PURPOSE: Host response to polytrauma occasionally has unpredictable outcomes. Immune response is a major factor influencing patient's outcome. This study evaluated the interaction of two main cytokines in immune response after major trauma, specifically interleukin-6 (IL-6) and interleukin-10 (...

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Autores principales: Sapan, Heber B., Paturusi, Idrus, Islam, Andi Asadul, Yusuf, Irawan, Patellongi, Ilhamjaya, Massi, Muhammmad Nasrum, Pusponegoro, Aryono D., Arief, Syafrie K., Labeda, Ibrahim, Rendy, Leo, Hatta, Mochammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832459/
https://www.ncbi.nlm.nih.gov/pubmed/29221655
http://dx.doi.org/10.1016/j.cjtee.2017.05.003
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author Sapan, Heber B.
Paturusi, Idrus
Islam, Andi Asadul
Yusuf, Irawan
Patellongi, Ilhamjaya
Massi, Muhammmad Nasrum
Pusponegoro, Aryono D.
Arief, Syafrie K.
Labeda, Ibrahim
Rendy, Leo
Hatta, Mochammad
author_facet Sapan, Heber B.
Paturusi, Idrus
Islam, Andi Asadul
Yusuf, Irawan
Patellongi, Ilhamjaya
Massi, Muhammmad Nasrum
Pusponegoro, Aryono D.
Arief, Syafrie K.
Labeda, Ibrahim
Rendy, Leo
Hatta, Mochammad
author_sort Sapan, Heber B.
collection PubMed
description PURPOSE: Host response to polytrauma occasionally has unpredictable outcomes. Immune response is a major factor influencing patient's outcome. This study evaluated the interaction of two main cytokines in immune response after major trauma, specifically interleukin-6 (IL-6) and interleukin-10 (IL-10). Plasma level of these cytokines is determined by mRNA expression of these cytokines genes which may decide the outcome of polytrauma patients. METHODS: This prospective multicenter trial held at four trauma centers enrolled 54 polytrauma patients [Injury Severity Score (ISS) ≥ 16]. Plasma levels and mRNA expression of IL-6 and IL-10 were measured for 5 days after trauma. Clinical evaluation was conducted to observe whether patients endured multiple organ dysfunction syndrome (MODS) and death. MODS evaluation was performed using sequential organ failure assessment (SOFA). Trauma load which in this study is represented with ISS, plasma level, expression of cytokine genes and patient's outcome were examined with correlation test and statistical analysis. RESULTS: The elevated IL-6/IL-10 ratio indicated increased activity of systemic inflammation response, especially pro-inflammation response which bears higher probability of progressing to MODS and death. The decline of IL-6/IL-10 ratio with heavy trauma load (ISS > 30) showed that compensatory anti-inflammation response syndrome (CARS) state was more dominant than systemic inflammatory response syndrome (SIRS), indicating that malfunction and failure of immune system eventually lead to MODS and deaths. The statistical significance in plasma level of cytokines was found in the outcome group which was defined as bearing a low trauma load but mortality. CONCLUSION: The pattern of cytokine levels in inflammation response has great impact on the outcome of polytrauma patients. Further study at the genetic level is needed to investigate inflammation process which may influence patient's outcome.
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spelling pubmed-58324592018-03-06 Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients Sapan, Heber B. Paturusi, Idrus Islam, Andi Asadul Yusuf, Irawan Patellongi, Ilhamjaya Massi, Muhammmad Nasrum Pusponegoro, Aryono D. Arief, Syafrie K. Labeda, Ibrahim Rendy, Leo Hatta, Mochammad Chin J Traumatol Original Article PURPOSE: Host response to polytrauma occasionally has unpredictable outcomes. Immune response is a major factor influencing patient's outcome. This study evaluated the interaction of two main cytokines in immune response after major trauma, specifically interleukin-6 (IL-6) and interleukin-10 (IL-10). Plasma level of these cytokines is determined by mRNA expression of these cytokines genes which may decide the outcome of polytrauma patients. METHODS: This prospective multicenter trial held at four trauma centers enrolled 54 polytrauma patients [Injury Severity Score (ISS) ≥ 16]. Plasma levels and mRNA expression of IL-6 and IL-10 were measured for 5 days after trauma. Clinical evaluation was conducted to observe whether patients endured multiple organ dysfunction syndrome (MODS) and death. MODS evaluation was performed using sequential organ failure assessment (SOFA). Trauma load which in this study is represented with ISS, plasma level, expression of cytokine genes and patient's outcome were examined with correlation test and statistical analysis. RESULTS: The elevated IL-6/IL-10 ratio indicated increased activity of systemic inflammation response, especially pro-inflammation response which bears higher probability of progressing to MODS and death. The decline of IL-6/IL-10 ratio with heavy trauma load (ISS > 30) showed that compensatory anti-inflammation response syndrome (CARS) state was more dominant than systemic inflammatory response syndrome (SIRS), indicating that malfunction and failure of immune system eventually lead to MODS and deaths. The statistical significance in plasma level of cytokines was found in the outcome group which was defined as bearing a low trauma load but mortality. CONCLUSION: The pattern of cytokine levels in inflammation response has great impact on the outcome of polytrauma patients. Further study at the genetic level is needed to investigate inflammation process which may influence patient's outcome. Elsevier 2017-12 2017-11-04 /pmc/articles/PMC5832459/ /pubmed/29221655 http://dx.doi.org/10.1016/j.cjtee.2017.05.003 Text en © 2017 Daping Hospital and the Research Institute of Surgery of the Third Military Medical University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Sapan, Heber B.
Paturusi, Idrus
Islam, Andi Asadul
Yusuf, Irawan
Patellongi, Ilhamjaya
Massi, Muhammmad Nasrum
Pusponegoro, Aryono D.
Arief, Syafrie K.
Labeda, Ibrahim
Rendy, Leo
Hatta, Mochammad
Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title_full Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title_fullStr Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title_full_unstemmed Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title_short Interleukin-6 and interleukin-10 plasma levels and mRNA expression in polytrauma patients
title_sort interleukin-6 and interleukin-10 plasma levels and mrna expression in polytrauma patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832459/
https://www.ncbi.nlm.nih.gov/pubmed/29221655
http://dx.doi.org/10.1016/j.cjtee.2017.05.003
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