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Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield

Rice yield potential has been stagnant since the Green Revolution in the late 1960s, especially in tropical rice cultivars. We evaluated the effect of two major genes that regulate grain number, Gn1a/OsCKX2 and IPA1/WFP/OsSPL14, in elite indica cultivar backgrounds. The yield-positive Gn1a-type 3 an...

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Autores principales: Kim, Sung-Ryul, Ramos, Joie M., Hizon, Rona Joy M., Ashikari, Motoyuki, Virk, Parminder S., Torres, Edgar A., Nissila, Eero, Jena, Kshirod K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832747/
https://www.ncbi.nlm.nih.gov/pubmed/29497052
http://dx.doi.org/10.1038/s41598-018-21355-4
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author Kim, Sung-Ryul
Ramos, Joie M.
Hizon, Rona Joy M.
Ashikari, Motoyuki
Virk, Parminder S.
Torres, Edgar A.
Nissila, Eero
Jena, Kshirod K.
author_facet Kim, Sung-Ryul
Ramos, Joie M.
Hizon, Rona Joy M.
Ashikari, Motoyuki
Virk, Parminder S.
Torres, Edgar A.
Nissila, Eero
Jena, Kshirod K.
author_sort Kim, Sung-Ryul
collection PubMed
description Rice yield potential has been stagnant since the Green Revolution in the late 1960s, especially in tropical rice cultivars. We evaluated the effect of two major genes that regulate grain number, Gn1a/OsCKX2 and IPA1/WFP/OsSPL14, in elite indica cultivar backgrounds. The yield-positive Gn1a-type 3 and OsSPL14(WFP) alleles were introgressed respectively through marker-assisted selection (MAS). The grain numbers per panicle (GNPP) were compared between the recipient allele and the donor allele groups using segregating plants in BC(3)F(2) and BC(3)F(3) generations. There was no significant difference in GNPP between the two Gn1a alleles, suggesting that the Gn1a-type 3 allele was not effective in indica cultivars. However, the OsSPL14(WFP) allele dramatically increased GNPP by 10.6–59.3% in all four different backgrounds across cropping seasons and generations, indicating that this allele provides strong genetic gain to elite indica cultivars. Eventually, five high-yielding breeding lines were bred using the OsSPL14(WFP) allele by MAS with a conventional breeding approach that showed increased grain yield by 28.4–83.5% (7.87–12.89 t/ha) vis-à-vis the recipient cultivars and exhibited higher yield (~64.7%) than the top-yielding check cultivar, IRRI 156 (7.82 t/ha). We demonstrated a strong possibility to increase the genetic yield potential of indica rice varieties through allele mining and its application.
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spelling pubmed-58327472018-03-05 Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield Kim, Sung-Ryul Ramos, Joie M. Hizon, Rona Joy M. Ashikari, Motoyuki Virk, Parminder S. Torres, Edgar A. Nissila, Eero Jena, Kshirod K. Sci Rep Article Rice yield potential has been stagnant since the Green Revolution in the late 1960s, especially in tropical rice cultivars. We evaluated the effect of two major genes that regulate grain number, Gn1a/OsCKX2 and IPA1/WFP/OsSPL14, in elite indica cultivar backgrounds. The yield-positive Gn1a-type 3 and OsSPL14(WFP) alleles were introgressed respectively through marker-assisted selection (MAS). The grain numbers per panicle (GNPP) were compared between the recipient allele and the donor allele groups using segregating plants in BC(3)F(2) and BC(3)F(3) generations. There was no significant difference in GNPP between the two Gn1a alleles, suggesting that the Gn1a-type 3 allele was not effective in indica cultivars. However, the OsSPL14(WFP) allele dramatically increased GNPP by 10.6–59.3% in all four different backgrounds across cropping seasons and generations, indicating that this allele provides strong genetic gain to elite indica cultivars. Eventually, five high-yielding breeding lines were bred using the OsSPL14(WFP) allele by MAS with a conventional breeding approach that showed increased grain yield by 28.4–83.5% (7.87–12.89 t/ha) vis-à-vis the recipient cultivars and exhibited higher yield (~64.7%) than the top-yielding check cultivar, IRRI 156 (7.82 t/ha). We demonstrated a strong possibility to increase the genetic yield potential of indica rice varieties through allele mining and its application. Nature Publishing Group UK 2018-03-01 /pmc/articles/PMC5832747/ /pubmed/29497052 http://dx.doi.org/10.1038/s41598-018-21355-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Sung-Ryul
Ramos, Joie M.
Hizon, Rona Joy M.
Ashikari, Motoyuki
Virk, Parminder S.
Torres, Edgar A.
Nissila, Eero
Jena, Kshirod K.
Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title_full Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title_fullStr Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title_full_unstemmed Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title_short Introgression of a functional epigenetic OsSPL14(WFP) allele into elite indica rice genomes greatly improved panicle traits and grain yield
title_sort introgression of a functional epigenetic osspl14(wfp) allele into elite indica rice genomes greatly improved panicle traits and grain yield
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832747/
https://www.ncbi.nlm.nih.gov/pubmed/29497052
http://dx.doi.org/10.1038/s41598-018-21355-4
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