ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes

Natural killer (NK) cells through their NK group 2 member D (NKG2D) receptors recognize NKG2D ligands such as UL16‐binding proteins (ULBPs) on virus‐infected cells and subsequently trigger the host innate immune response. In the present study, we demonstrated that hepatitis C virus (HCV) induced the...

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Autores principales: Dansako, Hiromichi, Imai, Hirotaka, Ueda, Youki, Satoh, Shinya, Wakita, Takaji, Kato, Nobuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832972/
https://www.ncbi.nlm.nih.gov/pubmed/29511613
http://dx.doi.org/10.1002/2211-5463.12373
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author Dansako, Hiromichi
Imai, Hirotaka
Ueda, Youki
Satoh, Shinya
Wakita, Takaji
Kato, Nobuyuki
author_facet Dansako, Hiromichi
Imai, Hirotaka
Ueda, Youki
Satoh, Shinya
Wakita, Takaji
Kato, Nobuyuki
author_sort Dansako, Hiromichi
collection PubMed
description Natural killer (NK) cells through their NK group 2 member D (NKG2D) receptors recognize NKG2D ligands such as UL16‐binding proteins (ULBPs) on virus‐infected cells and subsequently trigger the host innate immune response. In the present study, we demonstrated that hepatitis C virus (HCV) induced the cell surface expression of ULBP1 in human immortalized hepatocyte PH5CH8 cells and human hepatoma HuH‐7 cell‐derived RSc cells. Interestingly, NK cell line NK‐92 induced cytotoxicity and interferon‐γ mRNA expression and subsequently reduced the levels of HCV RNA replication during co‐culture with HCV‐infected RSc cells. From these results, we conclude that ULBP1 is a target of the NK cell‐mediated innate immune response in HCV‐infected human hepatocytes.
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spelling pubmed-58329722018-03-06 ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes Dansako, Hiromichi Imai, Hirotaka Ueda, Youki Satoh, Shinya Wakita, Takaji Kato, Nobuyuki FEBS Open Bio Research Articles Natural killer (NK) cells through their NK group 2 member D (NKG2D) receptors recognize NKG2D ligands such as UL16‐binding proteins (ULBPs) on virus‐infected cells and subsequently trigger the host innate immune response. In the present study, we demonstrated that hepatitis C virus (HCV) induced the cell surface expression of ULBP1 in human immortalized hepatocyte PH5CH8 cells and human hepatoma HuH‐7 cell‐derived RSc cells. Interestingly, NK cell line NK‐92 induced cytotoxicity and interferon‐γ mRNA expression and subsequently reduced the levels of HCV RNA replication during co‐culture with HCV‐infected RSc cells. From these results, we conclude that ULBP1 is a target of the NK cell‐mediated innate immune response in HCV‐infected human hepatocytes. John Wiley and Sons Inc. 2018-01-25 /pmc/articles/PMC5832972/ /pubmed/29511613 http://dx.doi.org/10.1002/2211-5463.12373 Text en © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Dansako, Hiromichi
Imai, Hirotaka
Ueda, Youki
Satoh, Shinya
Wakita, Takaji
Kato, Nobuyuki
ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title_full ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title_fullStr ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title_full_unstemmed ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title_short ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell‐mediated innate immune response in human hepatocytes
title_sort ulbp1 is induced by hepatitis c virus infection and is the target of the nk cell‐mediated innate immune response in human hepatocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5832972/
https://www.ncbi.nlm.nih.gov/pubmed/29511613
http://dx.doi.org/10.1002/2211-5463.12373
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