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Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses

In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated epithelial cell line (GEE), derived from embryonic goose tissue. The purified GEE cell line can efficiently grow over 65 passages in the M199 medium supplemented with 10% fetal bovine ser...

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Autores principales: Wang, Wenxiu, Said, Abdelrahman, Wang, Baoqin, Qu, Guanggang, Xu, Qingqing, Liu, Bo, Shen, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833280/
https://www.ncbi.nlm.nih.gov/pubmed/29494688
http://dx.doi.org/10.1371/journal.pone.0193876
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author Wang, Wenxiu
Said, Abdelrahman
Wang, Baoqin
Qu, Guanggang
Xu, Qingqing
Liu, Bo
Shen, Zhiqiang
author_facet Wang, Wenxiu
Said, Abdelrahman
Wang, Baoqin
Qu, Guanggang
Xu, Qingqing
Liu, Bo
Shen, Zhiqiang
author_sort Wang, Wenxiu
collection PubMed
description In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated epithelial cell line (GEE), derived from embryonic goose tissue. The purified GEE cell line can efficiently grow over 65 passages in the M199 medium supplemented with 10% fetal bovine serum at 37°C. Immunofluorescence assay was used to identify purified GEE cells as epithelial cell line by detecting expression of the Keratin-18 and -19. Further characterizations demonstrated that the GEE cell line can be continuously subcultured with (i) a high capacity to replicate for over 65 passages, (ii) a spontaneous epithelial-like morphology, (iii) constant chromosomal features and (iv) without an evidence of converting to tumorigenic cells either in vitro or in vivo study. Moreover, the GEE cell line can be effectively transfected with plasmids expressing reporter genes of different avian viruses, such as VP3, VP1 and F of goose parvo virus (GPV), duck hepatitis virus (DHV), and Newcastle disease virus (NDV), respectively. Finally, the established GEE cell line was evaluated for avian viruses infection susceptibility. Our results showed that the tested GPV, DHAV and NDV were capable to replicate in the new cell line with titers a comparatively higher to the ones detected in the traditional culture system. Accordingly, our established GEE cell line is apparently a suitable in vitro model for transgenic, and infection manipulation studies.
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spelling pubmed-58332802018-03-23 Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses Wang, Wenxiu Said, Abdelrahman Wang, Baoqin Qu, Guanggang Xu, Qingqing Liu, Bo Shen, Zhiqiang PLoS One Research Article In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated epithelial cell line (GEE), derived from embryonic goose tissue. The purified GEE cell line can efficiently grow over 65 passages in the M199 medium supplemented with 10% fetal bovine serum at 37°C. Immunofluorescence assay was used to identify purified GEE cells as epithelial cell line by detecting expression of the Keratin-18 and -19. Further characterizations demonstrated that the GEE cell line can be continuously subcultured with (i) a high capacity to replicate for over 65 passages, (ii) a spontaneous epithelial-like morphology, (iii) constant chromosomal features and (iv) without an evidence of converting to tumorigenic cells either in vitro or in vivo study. Moreover, the GEE cell line can be effectively transfected with plasmids expressing reporter genes of different avian viruses, such as VP3, VP1 and F of goose parvo virus (GPV), duck hepatitis virus (DHV), and Newcastle disease virus (NDV), respectively. Finally, the established GEE cell line was evaluated for avian viruses infection susceptibility. Our results showed that the tested GPV, DHAV and NDV were capable to replicate in the new cell line with titers a comparatively higher to the ones detected in the traditional culture system. Accordingly, our established GEE cell line is apparently a suitable in vitro model for transgenic, and infection manipulation studies. Public Library of Science 2018-03-01 /pmc/articles/PMC5833280/ /pubmed/29494688 http://dx.doi.org/10.1371/journal.pone.0193876 Text en © 2018 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Wenxiu
Said, Abdelrahman
Wang, Baoqin
Qu, Guanggang
Xu, Qingqing
Liu, Bo
Shen, Zhiqiang
Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title_full Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title_fullStr Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title_full_unstemmed Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title_short Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses
title_sort establishment and evaluation of the goose embryo epithelial (gee) cell line as a new model for propagation of avian viruses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833280/
https://www.ncbi.nlm.nih.gov/pubmed/29494688
http://dx.doi.org/10.1371/journal.pone.0193876
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