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Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells

AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the...

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Autores principales: Marie, Mélanie, Bigot, Karine, Angebault, Claire, Barrau, Coralie, Gondouin, Pauline, Pagan, Delphine, Fouquet, Stéphane, Villette, Thierry, Sahel, José-Alain, Lenaers, Guy, Picaud, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833722/
https://www.ncbi.nlm.nih.gov/pubmed/29459695
http://dx.doi.org/10.1038/s41419-018-0331-5
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author Marie, Mélanie
Bigot, Karine
Angebault, Claire
Barrau, Coralie
Gondouin, Pauline
Pagan, Delphine
Fouquet, Stéphane
Villette, Thierry
Sahel, José-Alain
Lenaers, Guy
Picaud, Serge
author_facet Marie, Mélanie
Bigot, Karine
Angebault, Claire
Barrau, Coralie
Gondouin, Pauline
Pagan, Delphine
Fouquet, Stéphane
Villette, Thierry
Sahel, José-Alain
Lenaers, Guy
Picaud, Serge
author_sort Marie, Mélanie
collection PubMed
description AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the mechanisms underlying light-elicited toxicity. RESULTS: Primary retinal pigment epithelium cells were loaded with a retinal photosensitizer, AE2, to mimic aging. Using a custom-made illumination device that delivers 10 nm-wide light bands, we demonstrated that A2E-loaded RPE cells generated high levels of both hydrogen peroxide (H(2)O(2)) and superoxide anion (O(2)(•−)) when exposed to blue–violet light. In addition, they exhibited perinuclear clustering of mitochondria with a decrease of both their mitochondrial membrane potential and their respiratory activities. The increase of oxidative stress resulted in increased levels of the oxidized form of glutathione and decreased superoxide dismutase (SOD) and catalase activities. Furthermore, mRNA expression levels of the main antioxidant enzymes (SOD2, catalase, and GPX1) also decreased. CONCLUSIONS: Using an innovative illumination device, we measured the precise action spectrum of the oxidative stress mechanisms on A2E-loaded retinal pigment epithelium cells. We defined 415–455 nm blue–violet light, within the solar spectrum reaching the retina, to be the spectral band that generates the highest amount of reactive oxygen species and produces the highest level of mitochondrial dysfunction, explaining its toxic effect. This study further highlights the need to filter these wavelengths from the eyes of AMD patients.
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spelling pubmed-58337222018-03-06 Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells Marie, Mélanie Bigot, Karine Angebault, Claire Barrau, Coralie Gondouin, Pauline Pagan, Delphine Fouquet, Stéphane Villette, Thierry Sahel, José-Alain Lenaers, Guy Picaud, Serge Cell Death Dis Article AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the mechanisms underlying light-elicited toxicity. RESULTS: Primary retinal pigment epithelium cells were loaded with a retinal photosensitizer, AE2, to mimic aging. Using a custom-made illumination device that delivers 10 nm-wide light bands, we demonstrated that A2E-loaded RPE cells generated high levels of both hydrogen peroxide (H(2)O(2)) and superoxide anion (O(2)(•−)) when exposed to blue–violet light. In addition, they exhibited perinuclear clustering of mitochondria with a decrease of both their mitochondrial membrane potential and their respiratory activities. The increase of oxidative stress resulted in increased levels of the oxidized form of glutathione and decreased superoxide dismutase (SOD) and catalase activities. Furthermore, mRNA expression levels of the main antioxidant enzymes (SOD2, catalase, and GPX1) also decreased. CONCLUSIONS: Using an innovative illumination device, we measured the precise action spectrum of the oxidative stress mechanisms on A2E-loaded retinal pigment epithelium cells. We defined 415–455 nm blue–violet light, within the solar spectrum reaching the retina, to be the spectral band that generates the highest amount of reactive oxygen species and produces the highest level of mitochondrial dysfunction, explaining its toxic effect. This study further highlights the need to filter these wavelengths from the eyes of AMD patients. Nature Publishing Group UK 2018-02-19 /pmc/articles/PMC5833722/ /pubmed/29459695 http://dx.doi.org/10.1038/s41419-018-0331-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marie, Mélanie
Bigot, Karine
Angebault, Claire
Barrau, Coralie
Gondouin, Pauline
Pagan, Delphine
Fouquet, Stéphane
Villette, Thierry
Sahel, José-Alain
Lenaers, Guy
Picaud, Serge
Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title_full Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title_fullStr Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title_full_unstemmed Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title_short Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
title_sort light action spectrum on oxidative stress and mitochondrial damage in a2e-loaded retinal pigment epithelium cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833722/
https://www.ncbi.nlm.nih.gov/pubmed/29459695
http://dx.doi.org/10.1038/s41419-018-0331-5
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