Cargando…
Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells
AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833722/ https://www.ncbi.nlm.nih.gov/pubmed/29459695 http://dx.doi.org/10.1038/s41419-018-0331-5 |
_version_ | 1783303520653410304 |
---|---|
author | Marie, Mélanie Bigot, Karine Angebault, Claire Barrau, Coralie Gondouin, Pauline Pagan, Delphine Fouquet, Stéphane Villette, Thierry Sahel, José-Alain Lenaers, Guy Picaud, Serge |
author_facet | Marie, Mélanie Bigot, Karine Angebault, Claire Barrau, Coralie Gondouin, Pauline Pagan, Delphine Fouquet, Stéphane Villette, Thierry Sahel, José-Alain Lenaers, Guy Picaud, Serge |
author_sort | Marie, Mélanie |
collection | PubMed |
description | AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the mechanisms underlying light-elicited toxicity. RESULTS: Primary retinal pigment epithelium cells were loaded with a retinal photosensitizer, AE2, to mimic aging. Using a custom-made illumination device that delivers 10 nm-wide light bands, we demonstrated that A2E-loaded RPE cells generated high levels of both hydrogen peroxide (H(2)O(2)) and superoxide anion (O(2)(•−)) when exposed to blue–violet light. In addition, they exhibited perinuclear clustering of mitochondria with a decrease of both their mitochondrial membrane potential and their respiratory activities. The increase of oxidative stress resulted in increased levels of the oxidized form of glutathione and decreased superoxide dismutase (SOD) and catalase activities. Furthermore, mRNA expression levels of the main antioxidant enzymes (SOD2, catalase, and GPX1) also decreased. CONCLUSIONS: Using an innovative illumination device, we measured the precise action spectrum of the oxidative stress mechanisms on A2E-loaded retinal pigment epithelium cells. We defined 415–455 nm blue–violet light, within the solar spectrum reaching the retina, to be the spectral band that generates the highest amount of reactive oxygen species and produces the highest level of mitochondrial dysfunction, explaining its toxic effect. This study further highlights the need to filter these wavelengths from the eyes of AMD patients. |
format | Online Article Text |
id | pubmed-5833722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58337222018-03-06 Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells Marie, Mélanie Bigot, Karine Angebault, Claire Barrau, Coralie Gondouin, Pauline Pagan, Delphine Fouquet, Stéphane Villette, Thierry Sahel, José-Alain Lenaers, Guy Picaud, Serge Cell Death Dis Article AIMS: Blue light is an identified risk factor for age-related macular degeneration (AMD). We investigated oxidative stress markers and mitochondrial changes in A2E-loaded retinal pigment epithelium cells under the blue–green part of the solar spectrum that reaches the retina to better understand the mechanisms underlying light-elicited toxicity. RESULTS: Primary retinal pigment epithelium cells were loaded with a retinal photosensitizer, AE2, to mimic aging. Using a custom-made illumination device that delivers 10 nm-wide light bands, we demonstrated that A2E-loaded RPE cells generated high levels of both hydrogen peroxide (H(2)O(2)) and superoxide anion (O(2)(•−)) when exposed to blue–violet light. In addition, they exhibited perinuclear clustering of mitochondria with a decrease of both their mitochondrial membrane potential and their respiratory activities. The increase of oxidative stress resulted in increased levels of the oxidized form of glutathione and decreased superoxide dismutase (SOD) and catalase activities. Furthermore, mRNA expression levels of the main antioxidant enzymes (SOD2, catalase, and GPX1) also decreased. CONCLUSIONS: Using an innovative illumination device, we measured the precise action spectrum of the oxidative stress mechanisms on A2E-loaded retinal pigment epithelium cells. We defined 415–455 nm blue–violet light, within the solar spectrum reaching the retina, to be the spectral band that generates the highest amount of reactive oxygen species and produces the highest level of mitochondrial dysfunction, explaining its toxic effect. This study further highlights the need to filter these wavelengths from the eyes of AMD patients. Nature Publishing Group UK 2018-02-19 /pmc/articles/PMC5833722/ /pubmed/29459695 http://dx.doi.org/10.1038/s41419-018-0331-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Marie, Mélanie Bigot, Karine Angebault, Claire Barrau, Coralie Gondouin, Pauline Pagan, Delphine Fouquet, Stéphane Villette, Thierry Sahel, José-Alain Lenaers, Guy Picaud, Serge Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title | Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title_full | Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title_fullStr | Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title_full_unstemmed | Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title_short | Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells |
title_sort | light action spectrum on oxidative stress and mitochondrial damage in a2e-loaded retinal pigment epithelium cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833722/ https://www.ncbi.nlm.nih.gov/pubmed/29459695 http://dx.doi.org/10.1038/s41419-018-0331-5 |
work_keys_str_mv | AT mariemelanie lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT bigotkarine lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT angebaultclaire lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT barraucoralie lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT gondouinpauline lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT pagandelphine lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT fouquetstephane lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT villettethierry lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT saheljosealain lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT lenaersguy lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells AT picaudserge lightactionspectrumonoxidativestressandmitochondrialdamageina2eloadedretinalpigmentepitheliumcells |