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MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A
MicroRNAs (miRNA) are a class of small, noncoding RNA molecules that regulate the expression of target genes. miRNA dysregulation is involved in carcinogenesis and tumor progression. In this study, we identified microRNA-1253 (miR-1253) as being significantly down-regulated in non-small-cell lung ca...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833797/ https://www.ncbi.nlm.nih.gov/pubmed/29415994 http://dx.doi.org/10.1038/s41419-017-0218-x |
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author | Liu, Meiyue Zhang, Yue Zhang, Jie Cai, Haifeng Zhang, Chao Yang, Zhao Niu, Yi Wang, Huan Wei, Xiaomei Wang, Wei Gao, Peng Li, Hongmin Zhang, Jinghua Sun, Guogui |
author_facet | Liu, Meiyue Zhang, Yue Zhang, Jie Cai, Haifeng Zhang, Chao Yang, Zhao Niu, Yi Wang, Huan Wei, Xiaomei Wang, Wei Gao, Peng Li, Hongmin Zhang, Jinghua Sun, Guogui |
author_sort | Liu, Meiyue |
collection | PubMed |
description | MicroRNAs (miRNA) are a class of small, noncoding RNA molecules that regulate the expression of target genes. miRNA dysregulation is involved in carcinogenesis and tumor progression. In this study, we identified microRNA-1253 (miR-1253) as being significantly down-regulated in non-small-cell lung carcinoma (NSCLC) tissues and associated with advanced clinical stage, lymph node metastasis, and poor survival. The enhanced expression of miR-1253 significantly inhibited the proliferation, migration, and invasion of NSCLC cells in vitro. Bioinformatics analyses showed that miR-1253 directly targeted WNT5A (long isoform), which was confirmed using the dual-luciferase reporter assay. The inhibitory effects of miR-1253 on the growth and metastasis of NSCLC cells were attenuated and phenocopied by WNT5A (long) overexpression and knockdown, respectively. Consistent with the in vitro results, subcutaneous tumor and metastatic NSCLC mouse models showed that miR-1253 functions as a potent suppressor of NSCLC in vivo. Taken together, our findings indicated that miR-1253 inhibited the proliferation and metastasis of NSCLC cells by targeting WNT5A (long isoform) and provided new evidence of miR-1253 as a potential therapeutic target in NSCLC. |
format | Online Article Text |
id | pubmed-5833797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58337972018-03-06 MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A Liu, Meiyue Zhang, Yue Zhang, Jie Cai, Haifeng Zhang, Chao Yang, Zhao Niu, Yi Wang, Huan Wei, Xiaomei Wang, Wei Gao, Peng Li, Hongmin Zhang, Jinghua Sun, Guogui Cell Death Dis Article MicroRNAs (miRNA) are a class of small, noncoding RNA molecules that regulate the expression of target genes. miRNA dysregulation is involved in carcinogenesis and tumor progression. In this study, we identified microRNA-1253 (miR-1253) as being significantly down-regulated in non-small-cell lung carcinoma (NSCLC) tissues and associated with advanced clinical stage, lymph node metastasis, and poor survival. The enhanced expression of miR-1253 significantly inhibited the proliferation, migration, and invasion of NSCLC cells in vitro. Bioinformatics analyses showed that miR-1253 directly targeted WNT5A (long isoform), which was confirmed using the dual-luciferase reporter assay. The inhibitory effects of miR-1253 on the growth and metastasis of NSCLC cells were attenuated and phenocopied by WNT5A (long) overexpression and knockdown, respectively. Consistent with the in vitro results, subcutaneous tumor and metastatic NSCLC mouse models showed that miR-1253 functions as a potent suppressor of NSCLC in vivo. Taken together, our findings indicated that miR-1253 inhibited the proliferation and metastasis of NSCLC cells by targeting WNT5A (long isoform) and provided new evidence of miR-1253 as a potential therapeutic target in NSCLC. Nature Publishing Group UK 2018-02-07 /pmc/articles/PMC5833797/ /pubmed/29415994 http://dx.doi.org/10.1038/s41419-017-0218-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Meiyue Zhang, Yue Zhang, Jie Cai, Haifeng Zhang, Chao Yang, Zhao Niu, Yi Wang, Huan Wei, Xiaomei Wang, Wei Gao, Peng Li, Hongmin Zhang, Jinghua Sun, Guogui MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title | MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title_full | MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title_fullStr | MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title_full_unstemmed | MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title_short | MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A |
title_sort | microrna-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting wnt5a |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833797/ https://www.ncbi.nlm.nih.gov/pubmed/29415994 http://dx.doi.org/10.1038/s41419-017-0218-x |
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