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Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors

The global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. Knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes re...

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Autores principales: Nyasembe, Vincent O., Tchouassi, David P., Pirk, Christian W. W., Sole, Catherine L., Torto, Baldwyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834208/
https://www.ncbi.nlm.nih.gov/pubmed/29462150
http://dx.doi.org/10.1371/journal.pntd.0006185
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author Nyasembe, Vincent O.
Tchouassi, David P.
Pirk, Christian W. W.
Sole, Catherine L.
Torto, Baldwyn
author_facet Nyasembe, Vincent O.
Tchouassi, David P.
Pirk, Christian W. W.
Sole, Catherine L.
Torto, Baldwyn
author_sort Nyasembe, Vincent O.
collection PubMed
description The global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. Knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. However, the identity of plant species mosquitoes feed on in nature remains largely unknown. By testing the hypothesis about selectivity in plant feeding, we employed a DNA-based approach targeting trnH-psbA and matK genes and identified host plants of field-collected Afro-tropical mosquito vectors of dengue, Rift Valley fever and malaria being among the most important mosquito-borne diseases in East Africa. These included three plant species for Aedes aegypti (dengue), two for both Aedes mcintoshi and Aedes ochraceus (Rift Valley fever) and five for Anopheles gambiae (malaria). Since plant feeding is mediated by olfactory cues, we further sought to identify specific odor signatures that may modulate host plant location. Using coupled gas chromatography (GC)-electroantennographic detection, GC/mass spectrometry and electroantennogram analyses, we identified a total of 21 antennally-active components variably detected by Ae. aegypti, Ae. mcintoshi and An. gambiae from their respective host plants. Whereas Ae. aegypti predominantly detected benzenoids, Ae. mcintoshi detected mainly aldehydes while An. gambiae detected sesquiterpenes and alkenes. Interestingly, the monoterpenes β-myrcene and (E)-β-ocimene were consistently detected by all the mosquito species and present in all the identified host plants, suggesting that they may serve as signature cues in plant location. This study highlights the utility of molecular approaches in identifying specific vector-plant associations, which can be exploited in maximizing control strategies such as such as attractive toxic sugar bait and odor-bait technology.
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spelling pubmed-58342082018-03-23 Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors Nyasembe, Vincent O. Tchouassi, David P. Pirk, Christian W. W. Sole, Catherine L. Torto, Baldwyn PLoS Negl Trop Dis Research Article The global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. Knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. However, the identity of plant species mosquitoes feed on in nature remains largely unknown. By testing the hypothesis about selectivity in plant feeding, we employed a DNA-based approach targeting trnH-psbA and matK genes and identified host plants of field-collected Afro-tropical mosquito vectors of dengue, Rift Valley fever and malaria being among the most important mosquito-borne diseases in East Africa. These included three plant species for Aedes aegypti (dengue), two for both Aedes mcintoshi and Aedes ochraceus (Rift Valley fever) and five for Anopheles gambiae (malaria). Since plant feeding is mediated by olfactory cues, we further sought to identify specific odor signatures that may modulate host plant location. Using coupled gas chromatography (GC)-electroantennographic detection, GC/mass spectrometry and electroantennogram analyses, we identified a total of 21 antennally-active components variably detected by Ae. aegypti, Ae. mcintoshi and An. gambiae from their respective host plants. Whereas Ae. aegypti predominantly detected benzenoids, Ae. mcintoshi detected mainly aldehydes while An. gambiae detected sesquiterpenes and alkenes. Interestingly, the monoterpenes β-myrcene and (E)-β-ocimene were consistently detected by all the mosquito species and present in all the identified host plants, suggesting that they may serve as signature cues in plant location. This study highlights the utility of molecular approaches in identifying specific vector-plant associations, which can be exploited in maximizing control strategies such as such as attractive toxic sugar bait and odor-bait technology. Public Library of Science 2018-02-20 /pmc/articles/PMC5834208/ /pubmed/29462150 http://dx.doi.org/10.1371/journal.pntd.0006185 Text en © 2018 Nyasembe et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nyasembe, Vincent O.
Tchouassi, David P.
Pirk, Christian W. W.
Sole, Catherine L.
Torto, Baldwyn
Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title_full Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title_fullStr Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title_full_unstemmed Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title_short Host plant forensics and olfactory-based detection in Afro-tropical mosquito disease vectors
title_sort host plant forensics and olfactory-based detection in afro-tropical mosquito disease vectors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834208/
https://www.ncbi.nlm.nih.gov/pubmed/29462150
http://dx.doi.org/10.1371/journal.pntd.0006185
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