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A sleep state in Drosophila larvae required for neural stem cell proliferation

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis...

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Autores principales: Szuperak, Milan, Churgin, Matthew A, Borja, Austin J, Raizen, David M, Fang-Yen, Christopher, Kayser, Matthew S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834245/
https://www.ncbi.nlm.nih.gov/pubmed/29424688
http://dx.doi.org/10.7554/eLife.33220
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author Szuperak, Milan
Churgin, Matthew A
Borja, Austin J
Raizen, David M
Fang-Yen, Christopher
Kayser, Matthew S
author_facet Szuperak, Milan
Churgin, Matthew A
Borja, Austin J
Raizen, David M
Fang-Yen, Christopher
Kayser, Matthew S
author_sort Szuperak, Milan
collection PubMed
description Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.
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spelling pubmed-58342452018-03-05 A sleep state in Drosophila larvae required for neural stem cell proliferation Szuperak, Milan Churgin, Matthew A Borja, Austin J Raizen, David M Fang-Yen, Christopher Kayser, Matthew S eLife Neuroscience Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation. eLife Sciences Publications, Ltd 2018-02-09 /pmc/articles/PMC5834245/ /pubmed/29424688 http://dx.doi.org/10.7554/eLife.33220 Text en © 2018, Szuperak et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Szuperak, Milan
Churgin, Matthew A
Borja, Austin J
Raizen, David M
Fang-Yen, Christopher
Kayser, Matthew S
A sleep state in Drosophila larvae required for neural stem cell proliferation
title A sleep state in Drosophila larvae required for neural stem cell proliferation
title_full A sleep state in Drosophila larvae required for neural stem cell proliferation
title_fullStr A sleep state in Drosophila larvae required for neural stem cell proliferation
title_full_unstemmed A sleep state in Drosophila larvae required for neural stem cell proliferation
title_short A sleep state in Drosophila larvae required for neural stem cell proliferation
title_sort sleep state in drosophila larvae required for neural stem cell proliferation
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834245/
https://www.ncbi.nlm.nih.gov/pubmed/29424688
http://dx.doi.org/10.7554/eLife.33220
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