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Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris

Pemphigus vulgaris is an autoimmune blistering disease characterized by a loss of epidermal cell–cell adhesion caused by anti-desmoglein (Dsg) autoantibodies. The pathogenesis of PV remains unclear. However, abnormal frequency and function of Th2 cells are believed to contribute to PV. We investigat...

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Autores principales: Chen, Jianbo, Zhang, Yao, Liang, Yunsheng, Zhao, Ming, Long, Hai, Xiao, Rong, Wu, Haijing, Liao, Jieyue, Luo, Shuaihantian, Zhang, Guiying, Lu, Qianjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834247/
https://www.ncbi.nlm.nih.gov/pubmed/29541411
http://dx.doi.org/10.18632/oncotarget.24371
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author Chen, Jianbo
Zhang, Yao
Liang, Yunsheng
Zhao, Ming
Long, Hai
Xiao, Rong
Wu, Haijing
Liao, Jieyue
Luo, Shuaihantian
Zhang, Guiying
Lu, Qianjin
author_facet Chen, Jianbo
Zhang, Yao
Liang, Yunsheng
Zhao, Ming
Long, Hai
Xiao, Rong
Wu, Haijing
Liao, Jieyue
Luo, Shuaihantian
Zhang, Guiying
Lu, Qianjin
author_sort Chen, Jianbo
collection PubMed
description Pemphigus vulgaris is an autoimmune blistering disease characterized by a loss of epidermal cell–cell adhesion caused by anti-desmoglein (Dsg) autoantibodies. The pathogenesis of PV remains unclear. However, abnormal frequency and function of Th2 cells are believed to contribute to PV. We investigated Nr4a2, a transcription factor, which has been found to regulate T cell differentiation, for its association with Th2 cell differentiation and functions in PV. We found significantly decreased mRNA and protein levels of Nr4a2 in CD4+ T cells from patients with PV, compared with healthy control subjects. In addition, mRNA and protein levels of Nr4a2 in CD4+ T cells were inversely correlated with serum levels of IL-4 and IL-13 in patients with PV. Overexpression of Nr4a2 in CD4+ T cells from patients with PV significantly reduced the mRNA levels of GATA3, IL-4, and IL-13, while Nr4a2 siRNA treatment showed the reverse effects on the expression of these Th2-related cytokines and transcription factors. The data suggest that the altered level of Nr4a2 in CD4+ T cells is associated with the development of PV. Nr4a2 may contribute to the pathogenesis of PV by negatively regulating Th2 activity and secretion of Th2-related cytokines.
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spelling pubmed-58342472018-03-14 Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris Chen, Jianbo Zhang, Yao Liang, Yunsheng Zhao, Ming Long, Hai Xiao, Rong Wu, Haijing Liao, Jieyue Luo, Shuaihantian Zhang, Guiying Lu, Qianjin Oncotarget Research Paper Pemphigus vulgaris is an autoimmune blistering disease characterized by a loss of epidermal cell–cell adhesion caused by anti-desmoglein (Dsg) autoantibodies. The pathogenesis of PV remains unclear. However, abnormal frequency and function of Th2 cells are believed to contribute to PV. We investigated Nr4a2, a transcription factor, which has been found to regulate T cell differentiation, for its association with Th2 cell differentiation and functions in PV. We found significantly decreased mRNA and protein levels of Nr4a2 in CD4+ T cells from patients with PV, compared with healthy control subjects. In addition, mRNA and protein levels of Nr4a2 in CD4+ T cells were inversely correlated with serum levels of IL-4 and IL-13 in patients with PV. Overexpression of Nr4a2 in CD4+ T cells from patients with PV significantly reduced the mRNA levels of GATA3, IL-4, and IL-13, while Nr4a2 siRNA treatment showed the reverse effects on the expression of these Th2-related cytokines and transcription factors. The data suggest that the altered level of Nr4a2 in CD4+ T cells is associated with the development of PV. Nr4a2 may contribute to the pathogenesis of PV by negatively regulating Th2 activity and secretion of Th2-related cytokines. Impact Journals LLC 2018-02-01 /pmc/articles/PMC5834247/ /pubmed/29541411 http://dx.doi.org/10.18632/oncotarget.24371 Text en Copyright: © 2018 Chen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, Jianbo
Zhang, Yao
Liang, Yunsheng
Zhao, Ming
Long, Hai
Xiao, Rong
Wu, Haijing
Liao, Jieyue
Luo, Shuaihantian
Zhang, Guiying
Lu, Qianjin
Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title_full Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title_fullStr Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title_full_unstemmed Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title_short Regulatory effects of Nr4a2 on Th2 cells from patients with pemphigus vulgaris
title_sort regulatory effects of nr4a2 on th2 cells from patients with pemphigus vulgaris
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834247/
https://www.ncbi.nlm.nih.gov/pubmed/29541411
http://dx.doi.org/10.18632/oncotarget.24371
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