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Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea

Bacterial strains were isolated from the sediments of the Baltic Sea using ferulic acid, guaiacol or a lignin-rich softwood waste stream as substrate. In total nine isolates were obtained, five on ferulic acid, two on guaiacol and two on a lignin-rich softwood stream as a carbon source. Three of the...

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Autores principales: Ravi, Krithika, García-Hidalgo, Javier, Nöbel, Matthias, Gorwa-Grauslund, Marie F., Lidén, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834416/
https://www.ncbi.nlm.nih.gov/pubmed/29500726
http://dx.doi.org/10.1186/s13568-018-0563-x
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author Ravi, Krithika
García-Hidalgo, Javier
Nöbel, Matthias
Gorwa-Grauslund, Marie F.
Lidén, Gunnar
author_facet Ravi, Krithika
García-Hidalgo, Javier
Nöbel, Matthias
Gorwa-Grauslund, Marie F.
Lidén, Gunnar
author_sort Ravi, Krithika
collection PubMed
description Bacterial strains were isolated from the sediments of the Baltic Sea using ferulic acid, guaiacol or a lignin-rich softwood waste stream as substrate. In total nine isolates were obtained, five on ferulic acid, two on guaiacol and two on a lignin-rich softwood stream as a carbon source. Three of the isolates were found to be Pseudomonas sp. based on 16S rRNA sequencing. Among them, isolate 9.1, which showed the fastest growth in defined M9 medium, was tentatively identified as a Pseudomonas deceptionensis strain based on the gyrB sequencing. The growth of isolate 9.1 was further examined on six selected lignin model compounds (ferulate, p-coumarate, benzoate, syringate, vanillin and guaiacol) from different upper funneling aromatic pathways and was found able to grow on four out of these six compounds. No growth was detected on syringate and guaiacol. The highest specific growth and uptake rates were observed for benzoate (0.3 h(−1) and 4.2 mmol g(CDW)(−1) h(−1)) whereas the lowest were for the compounds from the coniferyl branch. Interestingly, several pathway intermediates were excreted during batch growth. Vanillyl alcohol was found to be excreted during growth on vanillin. Several other intermediates like cis,cis-muconate, catechol, vanillate and 4-hydroxybenzoate from the known bacterial catabolic pathways were excreted during growth on the model compounds. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0563-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-58344162018-03-14 Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea Ravi, Krithika García-Hidalgo, Javier Nöbel, Matthias Gorwa-Grauslund, Marie F. Lidén, Gunnar AMB Express Original Article Bacterial strains were isolated from the sediments of the Baltic Sea using ferulic acid, guaiacol or a lignin-rich softwood waste stream as substrate. In total nine isolates were obtained, five on ferulic acid, two on guaiacol and two on a lignin-rich softwood stream as a carbon source. Three of the isolates were found to be Pseudomonas sp. based on 16S rRNA sequencing. Among them, isolate 9.1, which showed the fastest growth in defined M9 medium, was tentatively identified as a Pseudomonas deceptionensis strain based on the gyrB sequencing. The growth of isolate 9.1 was further examined on six selected lignin model compounds (ferulate, p-coumarate, benzoate, syringate, vanillin and guaiacol) from different upper funneling aromatic pathways and was found able to grow on four out of these six compounds. No growth was detected on syringate and guaiacol. The highest specific growth and uptake rates were observed for benzoate (0.3 h(−1) and 4.2 mmol g(CDW)(−1) h(−1)) whereas the lowest were for the compounds from the coniferyl branch. Interestingly, several pathway intermediates were excreted during batch growth. Vanillyl alcohol was found to be excreted during growth on vanillin. Several other intermediates like cis,cis-muconate, catechol, vanillate and 4-hydroxybenzoate from the known bacterial catabolic pathways were excreted during growth on the model compounds. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0563-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-03-02 /pmc/articles/PMC5834416/ /pubmed/29500726 http://dx.doi.org/10.1186/s13568-018-0563-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Ravi, Krithika
García-Hidalgo, Javier
Nöbel, Matthias
Gorwa-Grauslund, Marie F.
Lidén, Gunnar
Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title_full Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title_fullStr Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title_full_unstemmed Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title_short Biological conversion of aromatic monolignol compounds by a Pseudomonas isolate from sediments of the Baltic Sea
title_sort biological conversion of aromatic monolignol compounds by a pseudomonas isolate from sediments of the baltic sea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834416/
https://www.ncbi.nlm.nih.gov/pubmed/29500726
http://dx.doi.org/10.1186/s13568-018-0563-x
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