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Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?

The use of disc diffusion susceptibility tests to determine the antibacterial activity of engineered nanoparticles (ENPs) is questionable because their low diffusivity practically prevents them from penetrating through the culture media. In this study, we investigate the ability of such a test, name...

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Autores principales: Kourmouli, Angeliki, Valenti, Marco, van Rijn, Erwin, Beaumont, Hubertus J. E., Kalantzi, Olga-Ioanna, Schmidt-Ott, Andreas, Biskos, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834581/
https://www.ncbi.nlm.nih.gov/pubmed/29527123
http://dx.doi.org/10.1007/s11051-018-4152-3
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author Kourmouli, Angeliki
Valenti, Marco
van Rijn, Erwin
Beaumont, Hubertus J. E.
Kalantzi, Olga-Ioanna
Schmidt-Ott, Andreas
Biskos, George
author_facet Kourmouli, Angeliki
Valenti, Marco
van Rijn, Erwin
Beaumont, Hubertus J. E.
Kalantzi, Olga-Ioanna
Schmidt-Ott, Andreas
Biskos, George
author_sort Kourmouli, Angeliki
collection PubMed
description The use of disc diffusion susceptibility tests to determine the antibacterial activity of engineered nanoparticles (ENPs) is questionable because their low diffusivity practically prevents them from penetrating through the culture media. In this study, we investigate the ability of such a test, namely the Kirby-Bauer disc diffusion test, to determine the antimicrobial activity of Au and Ag ENPs having diameters from 10 to 40 nm on Escherichia coli cultures. As anticipated, the tests did not show any antibacterial effects of Au nanoparticles (NPs) as a result of their negligible diffusivity through the culture media. Ag NPs on the other hand exhibited a strong antimicrobial activity that was independent of their size. Considering that Ag, in contrast to Au, dissolves upon oxidation and dilution in aqueous solutions, the apparent antibacterial behavior of Ag NPs is attributed to the ions they release. The Kirby-Bauer method, and other similar tests, can therefore be employed to probe the antimicrobial activity of ENPs related to their ability to release ions rather than to their unique size-dependent properties. [Figure: see text]
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spelling pubmed-58345812018-03-09 Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles? Kourmouli, Angeliki Valenti, Marco van Rijn, Erwin Beaumont, Hubertus J. E. Kalantzi, Olga-Ioanna Schmidt-Ott, Andreas Biskos, George J Nanopart Res Brief Communication The use of disc diffusion susceptibility tests to determine the antibacterial activity of engineered nanoparticles (ENPs) is questionable because their low diffusivity practically prevents them from penetrating through the culture media. In this study, we investigate the ability of such a test, namely the Kirby-Bauer disc diffusion test, to determine the antimicrobial activity of Au and Ag ENPs having diameters from 10 to 40 nm on Escherichia coli cultures. As anticipated, the tests did not show any antibacterial effects of Au nanoparticles (NPs) as a result of their negligible diffusivity through the culture media. Ag NPs on the other hand exhibited a strong antimicrobial activity that was independent of their size. Considering that Ag, in contrast to Au, dissolves upon oxidation and dilution in aqueous solutions, the apparent antibacterial behavior of Ag NPs is attributed to the ions they release. The Kirby-Bauer method, and other similar tests, can therefore be employed to probe the antimicrobial activity of ENPs related to their ability to release ions rather than to their unique size-dependent properties. [Figure: see text] Springer Netherlands 2018-03-02 2018 /pmc/articles/PMC5834581/ /pubmed/29527123 http://dx.doi.org/10.1007/s11051-018-4152-3 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Brief Communication
Kourmouli, Angeliki
Valenti, Marco
van Rijn, Erwin
Beaumont, Hubertus J. E.
Kalantzi, Olga-Ioanna
Schmidt-Ott, Andreas
Biskos, George
Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title_full Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title_fullStr Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title_full_unstemmed Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title_short Can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
title_sort can disc diffusion susceptibility tests assess the antimicrobial activity of engineered nanoparticles?
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834581/
https://www.ncbi.nlm.nih.gov/pubmed/29527123
http://dx.doi.org/10.1007/s11051-018-4152-3
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