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A CREB2-targeting microRNA is required for long-term memory after single-trial learning
Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834643/ https://www.ncbi.nlm.nih.gov/pubmed/29500383 http://dx.doi.org/10.1038/s41598-018-22278-w |
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author | Korneev, Sergei A. Vavoulis, Dimitris V. Naskar, Souvik Dyakonova, Varvara E. Kemenes, Ildikó Kemenes, György |
author_facet | Korneev, Sergei A. Vavoulis, Dimitris V. Naskar, Souvik Dyakonova, Varvara E. Kemenes, Ildikó Kemenes, György |
author_sort | Korneev, Sergei A. |
collection | PubMed |
description | Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this process. To test this hypothesis, first we constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of Lymnaea stagnalis subjected to a single conditioning trial. Then, by next generation sequencing of these libraries, we identified a specific pool of miRNAs regulated by training. Of these miRNAs, we focussed on Lym-miR-137 whose seed region shows perfect complementarity to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known memory repressor. We found that Lym-miR-137 was transiently up-regulated 1 h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2 mRNA in an identified neuron with an established role in LTM. Finally, using an in vivo loss-of-function approach we demonstrated that Lym-miR-137 is required for single-trial induced LTM. |
format | Online Article Text |
id | pubmed-5834643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58346432018-03-05 A CREB2-targeting microRNA is required for long-term memory after single-trial learning Korneev, Sergei A. Vavoulis, Dimitris V. Naskar, Souvik Dyakonova, Varvara E. Kemenes, Ildikó Kemenes, György Sci Rep Article Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this process. To test this hypothesis, first we constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of Lymnaea stagnalis subjected to a single conditioning trial. Then, by next generation sequencing of these libraries, we identified a specific pool of miRNAs regulated by training. Of these miRNAs, we focussed on Lym-miR-137 whose seed region shows perfect complementarity to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known memory repressor. We found that Lym-miR-137 was transiently up-regulated 1 h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2 mRNA in an identified neuron with an established role in LTM. Finally, using an in vivo loss-of-function approach we demonstrated that Lym-miR-137 is required for single-trial induced LTM. Nature Publishing Group UK 2018-03-02 /pmc/articles/PMC5834643/ /pubmed/29500383 http://dx.doi.org/10.1038/s41598-018-22278-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Korneev, Sergei A. Vavoulis, Dimitris V. Naskar, Souvik Dyakonova, Varvara E. Kemenes, Ildikó Kemenes, György A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title | A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title_full | A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title_fullStr | A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title_full_unstemmed | A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title_short | A CREB2-targeting microRNA is required for long-term memory after single-trial learning |
title_sort | creb2-targeting microrna is required for long-term memory after single-trial learning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834643/ https://www.ncbi.nlm.nih.gov/pubmed/29500383 http://dx.doi.org/10.1038/s41598-018-22278-w |
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