6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells

Gingerols, the pungent ingredients in ginger, are reported to possess a cholesterol-lowering activity. However, the underlying mechanism remains unclear. The present study was to investigate how 6-gingerol (6-GN), the most abundant gingerol in fresh ginger, regulates hepatic cholesterol metabolism....

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Autores principales: Li, Xiao, Guo, Jingting, Liang, Ning, Jiang, Xinwei, Song, Yuan, Ou, Shiyi, Hu, Yunfeng, Jiao, Rui, Bai, Weibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835308/
https://www.ncbi.nlm.nih.gov/pubmed/29535632
http://dx.doi.org/10.3389/fphar.2018.00159
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author Li, Xiao
Guo, Jingting
Liang, Ning
Jiang, Xinwei
Song, Yuan
Ou, Shiyi
Hu, Yunfeng
Jiao, Rui
Bai, Weibin
author_facet Li, Xiao
Guo, Jingting
Liang, Ning
Jiang, Xinwei
Song, Yuan
Ou, Shiyi
Hu, Yunfeng
Jiao, Rui
Bai, Weibin
author_sort Li, Xiao
collection PubMed
description Gingerols, the pungent ingredients in ginger, are reported to possess a cholesterol-lowering activity. However, the underlying mechanism remains unclear. The present study was to investigate how 6-gingerol (6-GN), the most abundant gingerol in fresh ginger, regulates hepatic cholesterol metabolism. HepG2 cells were incubated with various concentrations of 6-GN ranging from 50 to 200 μM for 24 h. Results showed that both cellular total cholesterol and free cholesterol decreased in a dose-dependent manner. Besides, 6-GN ranging from 100 to 200 μM increased the LDLR protein and uptake of fluorescent-labeled LDL. Moreover, the mRNA and protein expressions of cholesterol metabolism-related genes were also examined. It was found that 6-GN regulated cholesterol metabolism via up-regulation of LDLR through activation of SREBP2 as well as up-regulation of cholesterol efflux-related genes LXRα and ABCA1.
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spelling pubmed-58353082018-03-13 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells Li, Xiao Guo, Jingting Liang, Ning Jiang, Xinwei Song, Yuan Ou, Shiyi Hu, Yunfeng Jiao, Rui Bai, Weibin Front Pharmacol Pharmacology Gingerols, the pungent ingredients in ginger, are reported to possess a cholesterol-lowering activity. However, the underlying mechanism remains unclear. The present study was to investigate how 6-gingerol (6-GN), the most abundant gingerol in fresh ginger, regulates hepatic cholesterol metabolism. HepG2 cells were incubated with various concentrations of 6-GN ranging from 50 to 200 μM for 24 h. Results showed that both cellular total cholesterol and free cholesterol decreased in a dose-dependent manner. Besides, 6-GN ranging from 100 to 200 μM increased the LDLR protein and uptake of fluorescent-labeled LDL. Moreover, the mRNA and protein expressions of cholesterol metabolism-related genes were also examined. It was found that 6-GN regulated cholesterol metabolism via up-regulation of LDLR through activation of SREBP2 as well as up-regulation of cholesterol efflux-related genes LXRα and ABCA1. Frontiers Media S.A. 2018-02-27 /pmc/articles/PMC5835308/ /pubmed/29535632 http://dx.doi.org/10.3389/fphar.2018.00159 Text en Copyright © 2018 Li, Guo, Liang, Jiang, Song, Ou, Hu, Jiao and Bai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Li, Xiao
Guo, Jingting
Liang, Ning
Jiang, Xinwei
Song, Yuan
Ou, Shiyi
Hu, Yunfeng
Jiao, Rui
Bai, Weibin
6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title_full 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title_fullStr 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title_full_unstemmed 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title_short 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
title_sort 6-gingerol regulates hepatic cholesterol metabolism by up-regulation of ldlr and cholesterol efflux-related genes in hepg2 cells
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835308/
https://www.ncbi.nlm.nih.gov/pubmed/29535632
http://dx.doi.org/10.3389/fphar.2018.00159
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