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Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training
The striated activator of rho‐signaling (STARS) protein acts as a link between external stimuli and exercise adaptation such as muscle hypertrophy. However, the acute and long‐term adaptational response of STARS is still unclear. This study aimed at investigating the acute and long‐term endurance tr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835521/ https://www.ncbi.nlm.nih.gov/pubmed/29504288 http://dx.doi.org/10.14814/phy2.13624 |
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author | Reitzner, Stefan M. Norrbom, Jessica Sundberg, Carl Johan Gidlund, Eva‐Karin |
author_facet | Reitzner, Stefan M. Norrbom, Jessica Sundberg, Carl Johan Gidlund, Eva‐Karin |
author_sort | Reitzner, Stefan M. |
collection | PubMed |
description | The striated activator of rho‐signaling (STARS) protein acts as a link between external stimuli and exercise adaptation such as muscle hypertrophy. However, the acute and long‐term adaptational response of STARS is still unclear. This study aimed at investigating the acute and long‐term endurance training response on the mRNA and protein expression of STARS and its related upstream and downstream factors in human skeletal muscle. mRNA and protein levels of STARS and related factors were assessed in skeletal muscle of healthy young men and women following an acute bout of endurance exercise (n = 15) or 12 weeks of one‐legged training (n = 23). Muscle biopsies were obtained before (acute and long‐term), at 30 min, 2, and 6 h following acute exercise, and at 24 h following both acute exercise and long‐term training. Following acute exercise, STARS mRNA was significantly elevated 3.9‐fold at 30 min returning back to baseline 24 h after exercise. STARS protein levels were numerically but nonsignificantly increased 7.2‐fold at 24 h. No changes in STARS or ERR α mRNA or STARS protein expression were seen following long‐term training. PGC‐1α mRNA increased 1.7‐fold following long‐term training. MRTF‐A mRNA was increased both following acute exercise and long‐term training, in contrast to SRF mRNA and protein which did not change. STARS mRNA is acutely upregulated with exercise, but there is no cumulative effect to long‐term training as seen in PGC‐1α mRNA expression. Exercise intensity might play a role in manifestation of protein expression, suggesting a more complex regulation of STARS. |
format | Online Article Text |
id | pubmed-5835521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58355212018-03-07 Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training Reitzner, Stefan M. Norrbom, Jessica Sundberg, Carl Johan Gidlund, Eva‐Karin Physiol Rep Original Research The striated activator of rho‐signaling (STARS) protein acts as a link between external stimuli and exercise adaptation such as muscle hypertrophy. However, the acute and long‐term adaptational response of STARS is still unclear. This study aimed at investigating the acute and long‐term endurance training response on the mRNA and protein expression of STARS and its related upstream and downstream factors in human skeletal muscle. mRNA and protein levels of STARS and related factors were assessed in skeletal muscle of healthy young men and women following an acute bout of endurance exercise (n = 15) or 12 weeks of one‐legged training (n = 23). Muscle biopsies were obtained before (acute and long‐term), at 30 min, 2, and 6 h following acute exercise, and at 24 h following both acute exercise and long‐term training. Following acute exercise, STARS mRNA was significantly elevated 3.9‐fold at 30 min returning back to baseline 24 h after exercise. STARS protein levels were numerically but nonsignificantly increased 7.2‐fold at 24 h. No changes in STARS or ERR α mRNA or STARS protein expression were seen following long‐term training. PGC‐1α mRNA increased 1.7‐fold following long‐term training. MRTF‐A mRNA was increased both following acute exercise and long‐term training, in contrast to SRF mRNA and protein which did not change. STARS mRNA is acutely upregulated with exercise, but there is no cumulative effect to long‐term training as seen in PGC‐1α mRNA expression. Exercise intensity might play a role in manifestation of protein expression, suggesting a more complex regulation of STARS. John Wiley and Sons Inc. 2018-03-04 /pmc/articles/PMC5835521/ /pubmed/29504288 http://dx.doi.org/10.14814/phy2.13624 Text en © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Reitzner, Stefan M. Norrbom, Jessica Sundberg, Carl Johan Gidlund, Eva‐Karin Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title | Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title_full | Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title_fullStr | Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title_full_unstemmed | Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title_short | Expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
title_sort | expression of striated activator of rho‐signaling in human skeletal muscle following acute exercise and long‐term training |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835521/ https://www.ncbi.nlm.nih.gov/pubmed/29504288 http://dx.doi.org/10.14814/phy2.13624 |
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