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Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region

Harmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Cam...

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Autores principales: Walter, Juline M., Lopes, Fabyano A. C., Lopes-Ferreira, Mônica, Vidal, Lívia M., Leomil, Luciana, Melo, Fabiana, de Azevedo, Girlene S., Oliveira, Rossandra M. S., Medeiros, Alba J., Melo, Adriana S. O., De Rezende, Carlos E., Tanuri, Amilcar, Thompson, Fabiano L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835534/
https://www.ncbi.nlm.nih.gov/pubmed/29541063
http://dx.doi.org/10.3389/fmicb.2018.00176
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author Walter, Juline M.
Lopes, Fabyano A. C.
Lopes-Ferreira, Mônica
Vidal, Lívia M.
Leomil, Luciana
Melo, Fabiana
de Azevedo, Girlene S.
Oliveira, Rossandra M. S.
Medeiros, Alba J.
Melo, Adriana S. O.
De Rezende, Carlos E.
Tanuri, Amilcar
Thompson, Fabiano L.
author_facet Walter, Juline M.
Lopes, Fabyano A. C.
Lopes-Ferreira, Mônica
Vidal, Lívia M.
Leomil, Luciana
Melo, Fabiana
de Azevedo, Girlene S.
Oliveira, Rossandra M. S.
Medeiros, Alba J.
Melo, Adriana S. O.
De Rezende, Carlos E.
Tanuri, Amilcar
Thompson, Fabiano L.
author_sort Walter, Juline M.
collection PubMed
description Harmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Campina Grande reservoirs. Our approach included metagenomics, microbial abundance quantification, ELISA test for three cyanotoxins (microcystin, nodularins, and cylindrospermopsin), and in vivo ecotoxicological tests with zebrafish embryos. Cytometry analysis showed high cyanobacterial abundance, while metagenomics identified an average of 10.6% of cyanobacterial sequences, and demonstrated the presence of Microcystis, Cylindrospermopsis, and toxin coding genes in all ponds. Zebrafish embryos reared with pond water had high mortality and diverse malformations. Among the ponds analyzed, Araçagi showed the highest lethality (an average of 62.9 ± 0.8%), followed by Boqueirão (lethality average of 62.5 ± 0.8%). Here, we demonstrate that water from ponds undergoing extremely drought conditions have an abundance of potentially harmful cyanobacteria and their toxins. Our findings are consistent with a scenario in which polluted drinking water poses a great risk to human health.
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spelling pubmed-58355342018-03-14 Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region Walter, Juline M. Lopes, Fabyano A. C. Lopes-Ferreira, Mônica Vidal, Lívia M. Leomil, Luciana Melo, Fabiana de Azevedo, Girlene S. Oliveira, Rossandra M. S. Medeiros, Alba J. Melo, Adriana S. O. De Rezende, Carlos E. Tanuri, Amilcar Thompson, Fabiano L. Front Microbiol Microbiology Harmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Campina Grande reservoirs. Our approach included metagenomics, microbial abundance quantification, ELISA test for three cyanotoxins (microcystin, nodularins, and cylindrospermopsin), and in vivo ecotoxicological tests with zebrafish embryos. Cytometry analysis showed high cyanobacterial abundance, while metagenomics identified an average of 10.6% of cyanobacterial sequences, and demonstrated the presence of Microcystis, Cylindrospermopsis, and toxin coding genes in all ponds. Zebrafish embryos reared with pond water had high mortality and diverse malformations. Among the ponds analyzed, Araçagi showed the highest lethality (an average of 62.9 ± 0.8%), followed by Boqueirão (lethality average of 62.5 ± 0.8%). Here, we demonstrate that water from ponds undergoing extremely drought conditions have an abundance of potentially harmful cyanobacteria and their toxins. Our findings are consistent with a scenario in which polluted drinking water poses a great risk to human health. Frontiers Media S.A. 2018-02-28 /pmc/articles/PMC5835534/ /pubmed/29541063 http://dx.doi.org/10.3389/fmicb.2018.00176 Text en Copyright © 2018 Walter, Lopes, Lopes-Ferreira, Vidal, Leomil, Melo, de Azevedo, Oliveira, Medeiros, Melo, De Rezende, Tanuri and Thompson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Walter, Juline M.
Lopes, Fabyano A. C.
Lopes-Ferreira, Mônica
Vidal, Lívia M.
Leomil, Luciana
Melo, Fabiana
de Azevedo, Girlene S.
Oliveira, Rossandra M. S.
Medeiros, Alba J.
Melo, Adriana S. O.
De Rezende, Carlos E.
Tanuri, Amilcar
Thompson, Fabiano L.
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title_full Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title_fullStr Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title_full_unstemmed Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title_short Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
title_sort occurrence of harmful cyanobacteria in drinking water from a severely drought-impacted semi-arid region
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835534/
https://www.ncbi.nlm.nih.gov/pubmed/29541063
http://dx.doi.org/10.3389/fmicb.2018.00176
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