Cargando…
β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835718/ https://www.ncbi.nlm.nih.gov/pubmed/29358311 http://dx.doi.org/10.1042/BSR20171386 |
_version_ | 1783303854767472640 |
---|---|
author | Xue, Cheng Hong, Ling-Ling Lin, Jun-Sheng Yao, Xiang-Yang Wu, Ding-Hui Lin, Xiao-Ping Zhang, Jia-Min Zhang, Xiao-Bin Zeng, Yi-Ming |
author_facet | Xue, Cheng Hong, Ling-Ling Lin, Jun-Sheng Yao, Xiang-Yang Wu, Ding-Hui Lin, Xiao-Ping Zhang, Jia-Min Zhang, Xiao-Bin Zeng, Yi-Ming |
author_sort | Xue, Cheng |
collection | PubMed |
description | Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of this disease. The purpose of the present study is to find out the mechanism for this effect. We cultured fibroblasts from normal human airway tissues and human airway granulation tissues. These cells were cultured with 160 μg/ml normal saline (NS), different doses of β-elemene, or 10 ng/ml canonical Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1). The proliferation rate of cells and the expression of six molecules involved in canonical Wnt/β-catenin pathway, Wnt3a, glycogen synthase kinase-3β (GSK-3β), β-catenin, α-smooth muscle actin (α-SMA), transforming growth factor-β (TGF-β), and Collagen I (Col-I), were measured. At last, we used canonical Wnt/β-catenin pathway activator (LiCl) to further ascertain the mechanism of β-elemene. Canonical Wnt/β-catenin pathway is activated in human airway granulation fibroblasts. β-Elemene didn’t affect normal human airway fibroblasts; however, it had a dose–responsive inhibitive effect on the proliferation and expression of Wnt3a, non-active GSK-3β, β-catenin, α-SMA, TGF-β, and Col-I of human airway granulation fibroblasts. More importantly, it had the same effect on the expression and nuclear translocation of active β-catenin. All these effects were similar to 10 ng/ml DKK-1 and could be attenuated by 10 mM LiCl. Thus, β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. This pathway is possibly a promising target to treat benign tracheobronchial stenosis. |
format | Online Article Text |
id | pubmed-5835718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58357182018-03-20 β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway Xue, Cheng Hong, Ling-Ling Lin, Jun-Sheng Yao, Xiang-Yang Wu, Ding-Hui Lin, Xiao-Ping Zhang, Jia-Min Zhang, Xiao-Bin Zeng, Yi-Ming Biosci Rep Research Articles Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of this disease. The purpose of the present study is to find out the mechanism for this effect. We cultured fibroblasts from normal human airway tissues and human airway granulation tissues. These cells were cultured with 160 μg/ml normal saline (NS), different doses of β-elemene, or 10 ng/ml canonical Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1). The proliferation rate of cells and the expression of six molecules involved in canonical Wnt/β-catenin pathway, Wnt3a, glycogen synthase kinase-3β (GSK-3β), β-catenin, α-smooth muscle actin (α-SMA), transforming growth factor-β (TGF-β), and Collagen I (Col-I), were measured. At last, we used canonical Wnt/β-catenin pathway activator (LiCl) to further ascertain the mechanism of β-elemene. Canonical Wnt/β-catenin pathway is activated in human airway granulation fibroblasts. β-Elemene didn’t affect normal human airway fibroblasts; however, it had a dose–responsive inhibitive effect on the proliferation and expression of Wnt3a, non-active GSK-3β, β-catenin, α-SMA, TGF-β, and Col-I of human airway granulation fibroblasts. More importantly, it had the same effect on the expression and nuclear translocation of active β-catenin. All these effects were similar to 10 ng/ml DKK-1 and could be attenuated by 10 mM LiCl. Thus, β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. This pathway is possibly a promising target to treat benign tracheobronchial stenosis. Portland Press Ltd. 2018-04-13 /pmc/articles/PMC5835718/ /pubmed/29358311 http://dx.doi.org/10.1042/BSR20171386 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Xue, Cheng Hong, Ling-Ling Lin, Jun-Sheng Yao, Xiang-Yang Wu, Ding-Hui Lin, Xiao-Ping Zhang, Jia-Min Zhang, Xiao-Bin Zeng, Yi-Ming β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title | β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title_full | β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title_fullStr | β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title_full_unstemmed | β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title_short | β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway |
title_sort | β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical wnt/β-catenin pathway |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835718/ https://www.ncbi.nlm.nih.gov/pubmed/29358311 http://dx.doi.org/10.1042/BSR20171386 |
work_keys_str_mv | AT xuecheng belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT honglingling belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT linjunsheng belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT yaoxiangyang belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT wudinghui belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT linxiaoping belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT zhangjiamin belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT zhangxiaobin belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway AT zengyiming belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway |