Cargando…

β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway

Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, Cheng, Hong, Ling-Ling, Lin, Jun-Sheng, Yao, Xiang-Yang, Wu, Ding-Hui, Lin, Xiao-Ping, Zhang, Jia-Min, Zhang, Xiao-Bin, Zeng, Yi-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835718/
https://www.ncbi.nlm.nih.gov/pubmed/29358311
http://dx.doi.org/10.1042/BSR20171386
_version_ 1783303854767472640
author Xue, Cheng
Hong, Ling-Ling
Lin, Jun-Sheng
Yao, Xiang-Yang
Wu, Ding-Hui
Lin, Xiao-Ping
Zhang, Jia-Min
Zhang, Xiao-Bin
Zeng, Yi-Ming
author_facet Xue, Cheng
Hong, Ling-Ling
Lin, Jun-Sheng
Yao, Xiang-Yang
Wu, Ding-Hui
Lin, Xiao-Ping
Zhang, Jia-Min
Zhang, Xiao-Bin
Zeng, Yi-Ming
author_sort Xue, Cheng
collection PubMed
description Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of this disease. The purpose of the present study is to find out the mechanism for this effect. We cultured fibroblasts from normal human airway tissues and human airway granulation tissues. These cells were cultured with 160 μg/ml normal saline (NS), different doses of β-elemene, or 10 ng/ml canonical Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1). The proliferation rate of cells and the expression of six molecules involved in canonical Wnt/β-catenin pathway, Wnt3a, glycogen synthase kinase-3β (GSK-3β), β-catenin, α-smooth muscle actin (α-SMA), transforming growth factor-β (TGF-β), and Collagen I (Col-I), were measured. At last, we used canonical Wnt/β-catenin pathway activator (LiCl) to further ascertain the mechanism of β-elemene. Canonical Wnt/β-catenin pathway is activated in human airway granulation fibroblasts. β-Elemene didn’t affect normal human airway fibroblasts; however, it had a dose–responsive inhibitive effect on the proliferation and expression of Wnt3a, non-active GSK-3β, β-catenin, α-SMA, TGF-β, and Col-I of human airway granulation fibroblasts. More importantly, it had the same effect on the expression and nuclear translocation of active β-catenin. All these effects were similar to 10 ng/ml DKK-1 and could be attenuated by 10 mM LiCl. Thus, β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. This pathway is possibly a promising target to treat benign tracheobronchial stenosis.
format Online
Article
Text
id pubmed-5835718
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-58357182018-03-20 β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway Xue, Cheng Hong, Ling-Ling Lin, Jun-Sheng Yao, Xiang-Yang Wu, Ding-Hui Lin, Xiao-Ping Zhang, Jia-Min Zhang, Xiao-Bin Zeng, Yi-Ming Biosci Rep Research Articles Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of this disease. The purpose of the present study is to find out the mechanism for this effect. We cultured fibroblasts from normal human airway tissues and human airway granulation tissues. These cells were cultured with 160 μg/ml normal saline (NS), different doses of β-elemene, or 10 ng/ml canonical Wnt/β-catenin pathway inhibitor (Dickkopf-1, DKK-1). The proliferation rate of cells and the expression of six molecules involved in canonical Wnt/β-catenin pathway, Wnt3a, glycogen synthase kinase-3β (GSK-3β), β-catenin, α-smooth muscle actin (α-SMA), transforming growth factor-β (TGF-β), and Collagen I (Col-I), were measured. At last, we used canonical Wnt/β-catenin pathway activator (LiCl) to further ascertain the mechanism of β-elemene. Canonical Wnt/β-catenin pathway is activated in human airway granulation fibroblasts. β-Elemene didn’t affect normal human airway fibroblasts; however, it had a dose–responsive inhibitive effect on the proliferation and expression of Wnt3a, non-active GSK-3β, β-catenin, α-SMA, TGF-β, and Col-I of human airway granulation fibroblasts. More importantly, it had the same effect on the expression and nuclear translocation of active β-catenin. All these effects were similar to 10 ng/ml DKK-1 and could be attenuated by 10 mM LiCl. Thus, β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. This pathway is possibly a promising target to treat benign tracheobronchial stenosis. Portland Press Ltd. 2018-04-13 /pmc/articles/PMC5835718/ /pubmed/29358311 http://dx.doi.org/10.1042/BSR20171386 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Xue, Cheng
Hong, Ling-Ling
Lin, Jun-Sheng
Yao, Xiang-Yang
Wu, Ding-Hui
Lin, Xiao-Ping
Zhang, Jia-Min
Zhang, Xiao-Bin
Zeng, Yi-Ming
β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title_full β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title_fullStr β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title_full_unstemmed β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title_short β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
title_sort β-elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical wnt/β-catenin pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835718/
https://www.ncbi.nlm.nih.gov/pubmed/29358311
http://dx.doi.org/10.1042/BSR20171386
work_keys_str_mv AT xuecheng belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT honglingling belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT linjunsheng belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT yaoxiangyang belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT wudinghui belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT linxiaoping belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT zhangjiamin belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT zhangxiaobin belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway
AT zengyiming belemeneinhibitstheproliferationofprimaryhumanairwaygranulationfibroblastsbydownregulatingcanonicalwntbcateninpathway