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GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade

Intraperitoneal metastasis is a common occurrence and is usually involved in the poor prognosis of ovarian cancer. Its specific metastatic pattern implies that certain indispensable microenvironmental factors secreted in the peritoneal cavity can direct metastatic ovarian cancer cells to permissive...

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Autores principales: Yung, Mingo Ming-Ho, Tang, Hermit Wai-Man, Cai, Patty Chun-Hui, Leung, Thomas Ho-Yin, Ngu, Siew-Fei, Chan, Karen Kar-Loen, Xu, Dakang, Yang, Huijuan, Ngan, Hextan Yuen-Sheung, Chan, David Wai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835935/
https://www.ncbi.nlm.nih.gov/pubmed/29507619
http://dx.doi.org/10.7150/thno.22536
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author Yung, Mingo Ming-Ho
Tang, Hermit Wai-Man
Cai, Patty Chun-Hui
Leung, Thomas Ho-Yin
Ngu, Siew-Fei
Chan, Karen Kar-Loen
Xu, Dakang
Yang, Huijuan
Ngan, Hextan Yuen-Sheung
Chan, David Wai
author_facet Yung, Mingo Ming-Ho
Tang, Hermit Wai-Man
Cai, Patty Chun-Hui
Leung, Thomas Ho-Yin
Ngu, Siew-Fei
Chan, Karen Kar-Loen
Xu, Dakang
Yang, Huijuan
Ngan, Hextan Yuen-Sheung
Chan, David Wai
author_sort Yung, Mingo Ming-Ho
collection PubMed
description Intraperitoneal metastasis is a common occurrence and is usually involved in the poor prognosis of ovarian cancer. Its specific metastatic pattern implies that certain indispensable microenvironmental factors secreted in the peritoneal cavity can direct metastatic ovarian cancer cells to permissive niches for secondary lesion formation. However, the underlying molecular mechanisms are ill defined. Herein, we report that GRO-α and IL-8 are predominately upregulated in culture media derived from either normal or cancerous omenta and are associated with increased ovarian cancer aggressiveness. Methods: OCM was established from culture medium of fresh human omental tissues. Primary and metastatic ovarian cancer cell lines were generated from human tumor tissues and verified by specific antibodies. The functional roles of GRO-α, IL-8, and their specific receptor CXCR2 were examined by neutralizing antibodies, shRNA gene knockdown, CRISPR/Cas9 gene knockout and pharmaceutical CXCR2 inhibitor SB225002. The oncogenic properties of ovarian cancer cells were examined by in vitro and in vivo mouse models. Results: Both GRO-α and IL-8 can activate TAK1/NFκB signaling via the CXCR2 receptor. Intriguingly, TAK1/NFκB signaling activity was higher in metastatic ovarian cancer cells; this higher activity makes them more susceptible to OCM-induced tumor aggressiveness. Treatment of ovarian cancer cells with GRO-α and IL-8 neutralizing antibodies or ablation of CXCR2 by shRNA gene knockdown, CRISPR/Cas9 gene knockout, or CXCR2 inhibitor SB225002 treatment significantly attenuated TAK1/NFκB signaling and decreased in vitro and in vivo oncogenic and metastatic potential, suggesting CXCR2 plays a key role in the GRO-α and IL-8-governed metastatic spreading of ovarian cancer cells in the intraperitoneal cavity. Conclusion: This study highlights the significance of GRO-α and IL-8 as the key chemokines in the peritoneal tumor microenvironment and suggests the utility of targeting their receptor CXCR2 as a potential target-based therapy for peritoneal metastases of ovarian cancer.
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spelling pubmed-58359352018-03-05 GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade Yung, Mingo Ming-Ho Tang, Hermit Wai-Man Cai, Patty Chun-Hui Leung, Thomas Ho-Yin Ngu, Siew-Fei Chan, Karen Kar-Loen Xu, Dakang Yang, Huijuan Ngan, Hextan Yuen-Sheung Chan, David Wai Theranostics Research Paper Intraperitoneal metastasis is a common occurrence and is usually involved in the poor prognosis of ovarian cancer. Its specific metastatic pattern implies that certain indispensable microenvironmental factors secreted in the peritoneal cavity can direct metastatic ovarian cancer cells to permissive niches for secondary lesion formation. However, the underlying molecular mechanisms are ill defined. Herein, we report that GRO-α and IL-8 are predominately upregulated in culture media derived from either normal or cancerous omenta and are associated with increased ovarian cancer aggressiveness. Methods: OCM was established from culture medium of fresh human omental tissues. Primary and metastatic ovarian cancer cell lines were generated from human tumor tissues and verified by specific antibodies. The functional roles of GRO-α, IL-8, and their specific receptor CXCR2 were examined by neutralizing antibodies, shRNA gene knockdown, CRISPR/Cas9 gene knockout and pharmaceutical CXCR2 inhibitor SB225002. The oncogenic properties of ovarian cancer cells were examined by in vitro and in vivo mouse models. Results: Both GRO-α and IL-8 can activate TAK1/NFκB signaling via the CXCR2 receptor. Intriguingly, TAK1/NFκB signaling activity was higher in metastatic ovarian cancer cells; this higher activity makes them more susceptible to OCM-induced tumor aggressiveness. Treatment of ovarian cancer cells with GRO-α and IL-8 neutralizing antibodies or ablation of CXCR2 by shRNA gene knockdown, CRISPR/Cas9 gene knockout, or CXCR2 inhibitor SB225002 treatment significantly attenuated TAK1/NFκB signaling and decreased in vitro and in vivo oncogenic and metastatic potential, suggesting CXCR2 plays a key role in the GRO-α and IL-8-governed metastatic spreading of ovarian cancer cells in the intraperitoneal cavity. Conclusion: This study highlights the significance of GRO-α and IL-8 as the key chemokines in the peritoneal tumor microenvironment and suggests the utility of targeting their receptor CXCR2 as a potential target-based therapy for peritoneal metastases of ovarian cancer. Ivyspring International Publisher 2018-02-02 /pmc/articles/PMC5835935/ /pubmed/29507619 http://dx.doi.org/10.7150/thno.22536 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Yung, Mingo Ming-Ho
Tang, Hermit Wai-Man
Cai, Patty Chun-Hui
Leung, Thomas Ho-Yin
Ngu, Siew-Fei
Chan, Karen Kar-Loen
Xu, Dakang
Yang, Huijuan
Ngan, Hextan Yuen-Sheung
Chan, David Wai
GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title_full GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title_fullStr GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title_full_unstemmed GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title_short GRO-α and IL-8 enhance ovarian cancer metastatic potential via the CXCR2-mediated TAK1/NFκB signaling cascade
title_sort gro-α and il-8 enhance ovarian cancer metastatic potential via the cxcr2-mediated tak1/nfκb signaling cascade
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835935/
https://www.ncbi.nlm.nih.gov/pubmed/29507619
http://dx.doi.org/10.7150/thno.22536
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