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Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery

Increasing amounts of evidence support the role of inflammation in epilepsy. This study was done to evaluate serum follow-up of IL-1β and IL-6 levels, as well as their concentration in the neocortex, and the relationship of central inflammation with NF-κB and annexin V in drug-resistant temporal lob...

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Autores principales: Lorigados Pedre, Lourdes, Morales Chacón, Lilia M., Pavón Fuentes, Nancy, Robinson Agramonte, María de los A., Serrano Sánchez, Teresa, Cruz-Xenes, Rachel M., Hung, Mei-Li Díaz, Estupiñán Díaz, Bárbara, Báez Martín, Margarita M., Orozco-Suárez, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836004/
https://www.ncbi.nlm.nih.gov/pubmed/29401729
http://dx.doi.org/10.3390/bs8020021
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author Lorigados Pedre, Lourdes
Morales Chacón, Lilia M.
Pavón Fuentes, Nancy
Robinson Agramonte, María de los A.
Serrano Sánchez, Teresa
Cruz-Xenes, Rachel M.
Hung, Mei-Li Díaz
Estupiñán Díaz, Bárbara
Báez Martín, Margarita M.
Orozco-Suárez, Sandra
author_facet Lorigados Pedre, Lourdes
Morales Chacón, Lilia M.
Pavón Fuentes, Nancy
Robinson Agramonte, María de los A.
Serrano Sánchez, Teresa
Cruz-Xenes, Rachel M.
Hung, Mei-Li Díaz
Estupiñán Díaz, Bárbara
Báez Martín, Margarita M.
Orozco-Suárez, Sandra
author_sort Lorigados Pedre, Lourdes
collection PubMed
description Increasing amounts of evidence support the role of inflammation in epilepsy. This study was done to evaluate serum follow-up of IL-1β and IL-6 levels, as well as their concentration in the neocortex, and the relationship of central inflammation with NF-κB and annexin V in drug-resistant temporal lobe epileptic (DRTLE) patients submitted to surgical treatment. Peripheral and central levels of IL-1β and IL-6were measured by ELISA in 10 DRTLE patients. The sera from patients were taken before surgery, and 12 and 24 months after surgical treatment. The neocortical expression of NF-κB was evaluated by western blotting and annexin V co-localization with synaptophysin by immunohistochemistry. The neocortical tissues from five patients who died by non-neurological causes were used as control. Decreased serum levels of IL-1 and IL-6 were observed after surgery; at this time, 70% of patients were seizure-free. No values of IL-1 and IL-6 were detected in neocortical control tissue, whereas cytokine levels were evidenced in DRTLE. Increased NF-κB neocortex expression was found and the positive annexin V neurons were more obvious in the DRTLE tissue, correlating with IL-6 levels. The follow-up study confirmed that the inflammatory alterations disappeared one year after surgery, when the majority of patients were seizure-free, and the apoptotic death process correlated with inflammation.
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spelling pubmed-58360042018-03-07 Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery Lorigados Pedre, Lourdes Morales Chacón, Lilia M. Pavón Fuentes, Nancy Robinson Agramonte, María de los A. Serrano Sánchez, Teresa Cruz-Xenes, Rachel M. Hung, Mei-Li Díaz Estupiñán Díaz, Bárbara Báez Martín, Margarita M. Orozco-Suárez, Sandra Behav Sci (Basel) Article Increasing amounts of evidence support the role of inflammation in epilepsy. This study was done to evaluate serum follow-up of IL-1β and IL-6 levels, as well as their concentration in the neocortex, and the relationship of central inflammation with NF-κB and annexin V in drug-resistant temporal lobe epileptic (DRTLE) patients submitted to surgical treatment. Peripheral and central levels of IL-1β and IL-6were measured by ELISA in 10 DRTLE patients. The sera from patients were taken before surgery, and 12 and 24 months after surgical treatment. The neocortical expression of NF-κB was evaluated by western blotting and annexin V co-localization with synaptophysin by immunohistochemistry. The neocortical tissues from five patients who died by non-neurological causes were used as control. Decreased serum levels of IL-1 and IL-6 were observed after surgery; at this time, 70% of patients were seizure-free. No values of IL-1 and IL-6 were detected in neocortical control tissue, whereas cytokine levels were evidenced in DRTLE. Increased NF-κB neocortex expression was found and the positive annexin V neurons were more obvious in the DRTLE tissue, correlating with IL-6 levels. The follow-up study confirmed that the inflammatory alterations disappeared one year after surgery, when the majority of patients were seizure-free, and the apoptotic death process correlated with inflammation. MDPI 2018-02-05 /pmc/articles/PMC5836004/ /pubmed/29401729 http://dx.doi.org/10.3390/bs8020021 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lorigados Pedre, Lourdes
Morales Chacón, Lilia M.
Pavón Fuentes, Nancy
Robinson Agramonte, María de los A.
Serrano Sánchez, Teresa
Cruz-Xenes, Rachel M.
Hung, Mei-Li Díaz
Estupiñán Díaz, Bárbara
Báez Martín, Margarita M.
Orozco-Suárez, Sandra
Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title_full Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title_fullStr Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title_full_unstemmed Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title_short Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery
title_sort follow-up of peripheral il-1β and il-6 and relation with apoptotic death in drug-resistant temporal lobe epilepsy patients submitted to surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836004/
https://www.ncbi.nlm.nih.gov/pubmed/29401729
http://dx.doi.org/10.3390/bs8020021
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