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Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide

The aim of this study was to determine the blood ionized calcium (Ca) levels and acute-phase blood glucose kinetics in goats with mastitis induced by an intramammary challenge of lipopolysaccharide (LPS). Five goats were subjected to intramammary challenge of either LPS (10 µg) or saline (control)....

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Autores principales: SHINOZUKA, Yasunori, KAWAI, Kazuhiro, SATO, Reiichiro, HIGASHITANI, Akito, HAMAMOTO, Yoshiki, OKITA, Miki, ISOBE, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836759/
https://www.ncbi.nlm.nih.gov/pubmed/29311427
http://dx.doi.org/10.1292/jvms.17-0615
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author SHINOZUKA, Yasunori
KAWAI, Kazuhiro
SATO, Reiichiro
HIGASHITANI, Akito
HAMAMOTO, Yoshiki
OKITA, Miki
ISOBE, Naoki
author_facet SHINOZUKA, Yasunori
KAWAI, Kazuhiro
SATO, Reiichiro
HIGASHITANI, Akito
HAMAMOTO, Yoshiki
OKITA, Miki
ISOBE, Naoki
author_sort SHINOZUKA, Yasunori
collection PubMed
description The aim of this study was to determine the blood ionized calcium (Ca) levels and acute-phase blood glucose kinetics in goats with mastitis induced by an intramammary challenge of lipopolysaccharide (LPS). Five goats were subjected to intramammary challenge of either LPS (10 µg) or saline (control). Some clinical manifestations (rectal temperature, pulse rate, respiration rate, ruminal motility, physical activity, and dehydration) were observed, and blood was collected for the measurement of several parameters [ionized and total Ca levels, blood glucose level, pH, and white blood count (WBC)] at 0 (just before challenge), 1–4, 6, 8, 12 and 24 hr post-challenge in both the LPS and control phases. Milk was collected at 0 (just before challenge), 4, 8, 12 and 24 hr post-challenge to measure the somatic cell count (SCC) and N-acetyl-beta-D-glucosaminidase (NAGase) activity. In the LPS phase, increased rectal temperature, significantly decreased ionized Ca and total Ca levels and WBCs were observed compared with those at 0 hr, although there were no differences in all parameters between phases. LPS infusion significantly increased SCCs in milk and NAGase activity. The present results demonstrated that, during the acute phase of mastitis induced by intramammary challenge by LPS at a concentration sufficient to cause general symptoms in goats, a decreased blood ionized Ca level occurs, but not hypoglycemia.
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spelling pubmed-58367592018-03-09 Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide SHINOZUKA, Yasunori KAWAI, Kazuhiro SATO, Reiichiro HIGASHITANI, Akito HAMAMOTO, Yoshiki OKITA, Miki ISOBE, Naoki J Vet Med Sci Physiology The aim of this study was to determine the blood ionized calcium (Ca) levels and acute-phase blood glucose kinetics in goats with mastitis induced by an intramammary challenge of lipopolysaccharide (LPS). Five goats were subjected to intramammary challenge of either LPS (10 µg) or saline (control). Some clinical manifestations (rectal temperature, pulse rate, respiration rate, ruminal motility, physical activity, and dehydration) were observed, and blood was collected for the measurement of several parameters [ionized and total Ca levels, blood glucose level, pH, and white blood count (WBC)] at 0 (just before challenge), 1–4, 6, 8, 12 and 24 hr post-challenge in both the LPS and control phases. Milk was collected at 0 (just before challenge), 4, 8, 12 and 24 hr post-challenge to measure the somatic cell count (SCC) and N-acetyl-beta-D-glucosaminidase (NAGase) activity. In the LPS phase, increased rectal temperature, significantly decreased ionized Ca and total Ca levels and WBCs were observed compared with those at 0 hr, although there were no differences in all parameters between phases. LPS infusion significantly increased SCCs in milk and NAGase activity. The present results demonstrated that, during the acute phase of mastitis induced by intramammary challenge by LPS at a concentration sufficient to cause general symptoms in goats, a decreased blood ionized Ca level occurs, but not hypoglycemia. The Japanese Society of Veterinary Science 2018-01-02 2018-02 /pmc/articles/PMC5836759/ /pubmed/29311427 http://dx.doi.org/10.1292/jvms.17-0615 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Physiology
SHINOZUKA, Yasunori
KAWAI, Kazuhiro
SATO, Reiichiro
HIGASHITANI, Akito
HAMAMOTO, Yoshiki
OKITA, Miki
ISOBE, Naoki
Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title_full Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title_fullStr Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title_full_unstemmed Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title_short Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
title_sort blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836759/
https://www.ncbi.nlm.nih.gov/pubmed/29311427
http://dx.doi.org/10.1292/jvms.17-0615
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