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Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors

Cryptococcal infections are mainly caused by members of the Cryptococcus neoformans species complex (molecular types VNI, VNII, VNB, VNIV and the AD hybrid VNIII). PCR of the mating type loci and MLST typing using the ISHAM-MLST consensus scheme were used to evaluate the genetic relationship of 102...

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Autores principales: Andrade-Silva, Leonardo Euripedes, Ferreira-Paim, Kennio, Ferreira, Thatiana Bragine, Vilas-Boas, Anderson, Mora, Delio José, Manzato, Verônica Morais, Fonseca, Fernanda Machado, Buosi, Kelli, Andrade-Silva, Juliana, Prudente, Bruno da Silva, Araujo, Natalia Evelyn, Sales-Campos, Helioswilton, da Silva, Marcus Vinicius, Júnior, Virmondes Rodrigues, Meyer, Wieland, Silva-Vergara, Mario Léon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5837091/
https://www.ncbi.nlm.nih.gov/pubmed/29505557
http://dx.doi.org/10.1371/journal.pone.0193237
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author Andrade-Silva, Leonardo Euripedes
Ferreira-Paim, Kennio
Ferreira, Thatiana Bragine
Vilas-Boas, Anderson
Mora, Delio José
Manzato, Verônica Morais
Fonseca, Fernanda Machado
Buosi, Kelli
Andrade-Silva, Juliana
Prudente, Bruno da Silva
Araujo, Natalia Evelyn
Sales-Campos, Helioswilton
da Silva, Marcus Vinicius
Júnior, Virmondes Rodrigues
Meyer, Wieland
Silva-Vergara, Mario Léon
author_facet Andrade-Silva, Leonardo Euripedes
Ferreira-Paim, Kennio
Ferreira, Thatiana Bragine
Vilas-Boas, Anderson
Mora, Delio José
Manzato, Verônica Morais
Fonseca, Fernanda Machado
Buosi, Kelli
Andrade-Silva, Juliana
Prudente, Bruno da Silva
Araujo, Natalia Evelyn
Sales-Campos, Helioswilton
da Silva, Marcus Vinicius
Júnior, Virmondes Rodrigues
Meyer, Wieland
Silva-Vergara, Mario Léon
author_sort Andrade-Silva, Leonardo Euripedes
collection PubMed
description Cryptococcal infections are mainly caused by members of the Cryptococcus neoformans species complex (molecular types VNI, VNII, VNB, VNIV and the AD hybrid VNIII). PCR of the mating type loci and MLST typing using the ISHAM-MLST consensus scheme were used to evaluate the genetic relationship of 102 (63 clinical and 39 environmental) C. neoformans isolates from Uberaba, Brazil and to correlate the obtained genotypes with clinical, antifungal susceptibility and virulence factor data. All isolates were mating type alpha. MLST identified 12 known and five new sequence types (ST). Fourteen STs were identified within the VNI isolates, with ST93 (57/102, 56%) and ST77 (19/102, 19%) being the most prevalent. From the nine VNII isolates previously identify by URA5-RFLP only four (ST40) were confirmed by MLST. The remaining five grouped within the VNB clade in the phylogenetic analysis corresponding to the sequence type ST504. Other two environmental isolates also grouped within VNB clade with the new sequence type ST527. The four VNII/ST40 isolates were isolated from CSF. The two VNIV sequence types (ST11 and ST160) were isolated from blood cultures. Two of six patients evaluated with more than one isolates had mixed infections. Amongst the VNI isolates 4 populations were identified, which showed differences in their susceptibility profiles, clinical outcome and virulence factors. These results reinforce that ST93 is the most prevalent ST in HIV-infected patients in the Southeastern region of Brazil. The finding of the VNB molecular type amongst environmental Brazilian isolates highlights that this genotype is not restricted to the African continent.
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spelling pubmed-58370912018-03-19 Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors Andrade-Silva, Leonardo Euripedes Ferreira-Paim, Kennio Ferreira, Thatiana Bragine Vilas-Boas, Anderson Mora, Delio José Manzato, Verônica Morais Fonseca, Fernanda Machado Buosi, Kelli Andrade-Silva, Juliana Prudente, Bruno da Silva Araujo, Natalia Evelyn Sales-Campos, Helioswilton da Silva, Marcus Vinicius Júnior, Virmondes Rodrigues Meyer, Wieland Silva-Vergara, Mario Léon PLoS One Research Article Cryptococcal infections are mainly caused by members of the Cryptococcus neoformans species complex (molecular types VNI, VNII, VNB, VNIV and the AD hybrid VNIII). PCR of the mating type loci and MLST typing using the ISHAM-MLST consensus scheme were used to evaluate the genetic relationship of 102 (63 clinical and 39 environmental) C. neoformans isolates from Uberaba, Brazil and to correlate the obtained genotypes with clinical, antifungal susceptibility and virulence factor data. All isolates were mating type alpha. MLST identified 12 known and five new sequence types (ST). Fourteen STs were identified within the VNI isolates, with ST93 (57/102, 56%) and ST77 (19/102, 19%) being the most prevalent. From the nine VNII isolates previously identify by URA5-RFLP only four (ST40) were confirmed by MLST. The remaining five grouped within the VNB clade in the phylogenetic analysis corresponding to the sequence type ST504. Other two environmental isolates also grouped within VNB clade with the new sequence type ST527. The four VNII/ST40 isolates were isolated from CSF. The two VNIV sequence types (ST11 and ST160) were isolated from blood cultures. Two of six patients evaluated with more than one isolates had mixed infections. Amongst the VNI isolates 4 populations were identified, which showed differences in their susceptibility profiles, clinical outcome and virulence factors. These results reinforce that ST93 is the most prevalent ST in HIV-infected patients in the Southeastern region of Brazil. The finding of the VNB molecular type amongst environmental Brazilian isolates highlights that this genotype is not restricted to the African continent. Public Library of Science 2018-03-05 /pmc/articles/PMC5837091/ /pubmed/29505557 http://dx.doi.org/10.1371/journal.pone.0193237 Text en © 2018 Andrade-Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Andrade-Silva, Leonardo Euripedes
Ferreira-Paim, Kennio
Ferreira, Thatiana Bragine
Vilas-Boas, Anderson
Mora, Delio José
Manzato, Verônica Morais
Fonseca, Fernanda Machado
Buosi, Kelli
Andrade-Silva, Juliana
Prudente, Bruno da Silva
Araujo, Natalia Evelyn
Sales-Campos, Helioswilton
da Silva, Marcus Vinicius
Júnior, Virmondes Rodrigues
Meyer, Wieland
Silva-Vergara, Mario Léon
Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title_full Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title_fullStr Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title_full_unstemmed Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title_short Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors
title_sort genotypic analysis of clinical and environmental cryptococcus neoformans isolates from brazil reveals the presence of vnb isolates and a correlation with biological factors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5837091/
https://www.ncbi.nlm.nih.gov/pubmed/29505557
http://dx.doi.org/10.1371/journal.pone.0193237
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