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The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis
Inflammatory bowel disease encompasses a group of chronic-inflammatory conditions of the colon and small intestine. These conditions are characterized by exacerbated inflammation of the organ that greatly affects the quality of life of patients. Molecular mechanisms counteracting this hyperinflammat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5837963/ https://www.ncbi.nlm.nih.gov/pubmed/29545807 http://dx.doi.org/10.3389/fimmu.2018.00400 |
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author | Cardoso, Ana Gil Castro, Antonio Martins, Ana Catarina Carriche, Guilhermina M. Murigneux, Valentine Castro, Isabel Cumano, Ana Vieira, Paulo Saraiva, Margarida |
author_facet | Cardoso, Ana Gil Castro, Antonio Martins, Ana Catarina Carriche, Guilhermina M. Murigneux, Valentine Castro, Isabel Cumano, Ana Vieira, Paulo Saraiva, Margarida |
author_sort | Cardoso, Ana |
collection | PubMed |
description | Inflammatory bowel disease encompasses a group of chronic-inflammatory conditions of the colon and small intestine. These conditions are characterized by exacerbated inflammation of the organ that greatly affects the quality of life of patients. Molecular mechanisms counteracting this hyperinflammatory status of the gut offer strategies for therapeutic intervention. Among these regulatory molecules is the anti-inflammatory cytokine interleukin (IL)-10, as shown in mice and humans. Indeed, IL-10 signaling, particularly in macrophages, is essential for intestinal homeostasis. We sought to investigate the temporal profile of IL-10-mediated protection during chemical colitis and which were the underlying mechanisms. Using a novel mouse model of inducible IL-10 overexpression (pMT-10), described here, we show that mice preconditioned with IL-10 for 8 days before dextran sulfate sodium (DSS) administration developed a milder colitic phenotype. In IL-10-induced colitic mice, Ly6C cells isolated from the lamina propria showed a decreased inflammatory profile. Because our mouse model leads to transcription of the IL-10 transgene in the bone marrow and elevated seric IL-10 concentration, we investigated whether IL-10 could imprint immune cells in a long-lasting way, thus conferring sustained protection to colitis. We show that this was not the case, as IL-10-afforded protection was only observed if IL-10 induction immediately preceded DSS-mediated colitis. Thus, despite the protection afforded by IL-10 in colitis, novel strategies are required, specifically to achieve long-lasting protection. |
format | Online Article Text |
id | pubmed-5837963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58379632018-03-15 The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis Cardoso, Ana Gil Castro, Antonio Martins, Ana Catarina Carriche, Guilhermina M. Murigneux, Valentine Castro, Isabel Cumano, Ana Vieira, Paulo Saraiva, Margarida Front Immunol Immunology Inflammatory bowel disease encompasses a group of chronic-inflammatory conditions of the colon and small intestine. These conditions are characterized by exacerbated inflammation of the organ that greatly affects the quality of life of patients. Molecular mechanisms counteracting this hyperinflammatory status of the gut offer strategies for therapeutic intervention. Among these regulatory molecules is the anti-inflammatory cytokine interleukin (IL)-10, as shown in mice and humans. Indeed, IL-10 signaling, particularly in macrophages, is essential for intestinal homeostasis. We sought to investigate the temporal profile of IL-10-mediated protection during chemical colitis and which were the underlying mechanisms. Using a novel mouse model of inducible IL-10 overexpression (pMT-10), described here, we show that mice preconditioned with IL-10 for 8 days before dextran sulfate sodium (DSS) administration developed a milder colitic phenotype. In IL-10-induced colitic mice, Ly6C cells isolated from the lamina propria showed a decreased inflammatory profile. Because our mouse model leads to transcription of the IL-10 transgene in the bone marrow and elevated seric IL-10 concentration, we investigated whether IL-10 could imprint immune cells in a long-lasting way, thus conferring sustained protection to colitis. We show that this was not the case, as IL-10-afforded protection was only observed if IL-10 induction immediately preceded DSS-mediated colitis. Thus, despite the protection afforded by IL-10 in colitis, novel strategies are required, specifically to achieve long-lasting protection. Frontiers Media S.A. 2018-03-01 /pmc/articles/PMC5837963/ /pubmed/29545807 http://dx.doi.org/10.3389/fimmu.2018.00400 Text en Copyright © 2018 Cardoso, Gil Castro, Martins, Carriche, Murigneux, Castro, Cumano, Vieira and Saraiva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Cardoso, Ana Gil Castro, Antonio Martins, Ana Catarina Carriche, Guilhermina M. Murigneux, Valentine Castro, Isabel Cumano, Ana Vieira, Paulo Saraiva, Margarida The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title | The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title_full | The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title_fullStr | The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title_full_unstemmed | The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title_short | The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodium-Induced Colitis |
title_sort | dynamics of interleukin-10-afforded protection during dextran sulfate sodium-induced colitis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5837963/ https://www.ncbi.nlm.nih.gov/pubmed/29545807 http://dx.doi.org/10.3389/fimmu.2018.00400 |
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