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Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients

AIM: In this study, we determined the gene expression analysis of IL-17 gene family for early detection of subclinical inflammation among IBD patients. BACKGROUND: Cytokines have a vital role in the pathogenesis of inflammatory bowel disease (IBD). Interleukin-17 is the signature cytokine of the rec...

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Autores principales: Safari, Mohammad Taghi, Chaleshi, Vahid, Tarban, Peyman, Nourian, Mahyar, Balaii, Hedieh, Shahrokh, Shabnam, Asadzadeh Aghdaei, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838185/
https://www.ncbi.nlm.nih.gov/pubmed/29511476
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author Safari, Mohammad Taghi
Chaleshi, Vahid
Tarban, Peyman
Nourian, Mahyar
Balaii, Hedieh
Shahrokh, Shabnam
Asadzadeh Aghdaei, Hamid
author_facet Safari, Mohammad Taghi
Chaleshi, Vahid
Tarban, Peyman
Nourian, Mahyar
Balaii, Hedieh
Shahrokh, Shabnam
Asadzadeh Aghdaei, Hamid
author_sort Safari, Mohammad Taghi
collection PubMed
description AIM: In this study, we determined the gene expression analysis of IL-17 gene family for early detection of subclinical inflammation among IBD patients. BACKGROUND: Cytokines have a vital role in the pathogenesis of inflammatory bowel disease (IBD). Interleukin-17 is the signature cytokine of the recently identified T helper 17 (Th17) cell subset. IL-17F is mainly involved in mucosal host defense mechanisms whereas the functions of IL-17B remain largely elusive. METHODS: In this cross-sectional study, IBD patients divided into two active and inactive groups. Peripheral blood mononuclear cells (PBMCs) from 38 IBD patients which 20 inactive samples and 18 active individuals were collected. Changes of IL-17 F and IL-17B mRNA expression level evaluated by quantitative-real time-PCR. RESULTS: mRNA expression level of IL-17B and IL-17F in CD, UC, active and inactive groups have been assessed and there were no significant differences (P>0.05). Patients were classified into five different categories as follows: i) 5ASA; ii) 5ASA + Pred; iii) 5ASA + AZA; iv) 5ASA + Pred + AZA; v) 5ASA + Pred + AZA + IFX according to medication usage, expression of IL-17F and IL-17B had no differences (p>0.05). CONCLUSION: Evaluation of IL-17B and IL-17F mRNA expression level illustrate no difference among active and inactive patients. Therefore, IL-17B and IL-17F are not biomarkers in an Iranian IBD patients.
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spelling pubmed-58381852018-03-06 Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients Safari, Mohammad Taghi Chaleshi, Vahid Tarban, Peyman Nourian, Mahyar Balaii, Hedieh Shahrokh, Shabnam Asadzadeh Aghdaei, Hamid Gastroenterol Hepatol Bed Bench Original Article AIM: In this study, we determined the gene expression analysis of IL-17 gene family for early detection of subclinical inflammation among IBD patients. BACKGROUND: Cytokines have a vital role in the pathogenesis of inflammatory bowel disease (IBD). Interleukin-17 is the signature cytokine of the recently identified T helper 17 (Th17) cell subset. IL-17F is mainly involved in mucosal host defense mechanisms whereas the functions of IL-17B remain largely elusive. METHODS: In this cross-sectional study, IBD patients divided into two active and inactive groups. Peripheral blood mononuclear cells (PBMCs) from 38 IBD patients which 20 inactive samples and 18 active individuals were collected. Changes of IL-17 F and IL-17B mRNA expression level evaluated by quantitative-real time-PCR. RESULTS: mRNA expression level of IL-17B and IL-17F in CD, UC, active and inactive groups have been assessed and there were no significant differences (P>0.05). Patients were classified into five different categories as follows: i) 5ASA; ii) 5ASA + Pred; iii) 5ASA + AZA; iv) 5ASA + Pred + AZA; v) 5ASA + Pred + AZA + IFX according to medication usage, expression of IL-17F and IL-17B had no differences (p>0.05). CONCLUSION: Evaluation of IL-17B and IL-17F mRNA expression level illustrate no difference among active and inactive patients. Therefore, IL-17B and IL-17F are not biomarkers in an Iranian IBD patients. Shaheed Beheshti University of Medical Sciences 2017 /pmc/articles/PMC5838185/ /pubmed/29511476 Text en ©2017 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Safari, Mohammad Taghi
Chaleshi, Vahid
Tarban, Peyman
Nourian, Mahyar
Balaii, Hedieh
Shahrokh, Shabnam
Asadzadeh Aghdaei, Hamid
Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title_full Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title_fullStr Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title_full_unstemmed Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title_short Evaluation of IL-17B and IL-17F mRNA expression in peripheral blood mononuclear cells and association with clinical outcome of IBD patients
title_sort evaluation of il-17b and il-17f mrna expression in peripheral blood mononuclear cells and association with clinical outcome of ibd patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838185/
https://www.ncbi.nlm.nih.gov/pubmed/29511476
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