Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fruct...

Descripción completa

Detalles Bibliográficos
Autores principales: Lampard, Emma V., Sedgwick, Adam C., Sombuttan, Thitima, Williams, George T., Wannalerse, Boontana, Jenkins, A. Toby A., Bull, Steven D., James, Tony D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838390/
https://www.ncbi.nlm.nih.gov/pubmed/29531891
http://dx.doi.org/10.1002/open.201700193
Descripción
Sumario:Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

Ejemplares similares