Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fruct...

Descripción completa

Detalles Bibliográficos
Autores principales: Lampard, Emma V., Sedgwick, Adam C., Sombuttan, Thitima, Williams, George T., Wannalerse, Boontana, Jenkins, A. Toby A., Bull, Steven D., James, Tony D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838390/
https://www.ncbi.nlm.nih.gov/pubmed/29531891
http://dx.doi.org/10.1002/open.201700193
_version_ 1783304248481546240
author Lampard, Emma V.
Sedgwick, Adam C.
Sombuttan, Thitima
Williams, George T.
Wannalerse, Boontana
Jenkins, A. Toby A.
Bull, Steven D.
James, Tony D.
author_facet Lampard, Emma V.
Sedgwick, Adam C.
Sombuttan, Thitima
Williams, George T.
Wannalerse, Boontana
Jenkins, A. Toby A.
Bull, Steven D.
James, Tony D.
author_sort Lampard, Emma V.
collection PubMed
description Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.
format Online
Article
Text
id pubmed-5838390
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-58383902018-03-12 Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels Lampard, Emma V. Sedgwick, Adam C. Sombuttan, Thitima Williams, George T. Wannalerse, Boontana Jenkins, A. Toby A. Bull, Steven D. James, Tony D. ChemistryOpen Communications Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose. John Wiley and Sons Inc. 2018-01-30 /pmc/articles/PMC5838390/ /pubmed/29531891 http://dx.doi.org/10.1002/open.201700193 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Lampard, Emma V.
Sedgwick, Adam C.
Sombuttan, Thitima
Williams, George T.
Wannalerse, Boontana
Jenkins, A. Toby A.
Bull, Steven D.
James, Tony D.
Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title_full Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title_fullStr Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title_full_unstemmed Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title_short Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels
title_sort dye displacement assay for saccharides using benzoxaborole hydrogels
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838390/
https://www.ncbi.nlm.nih.gov/pubmed/29531891
http://dx.doi.org/10.1002/open.201700193
work_keys_str_mv AT lampardemmav dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT sedgwickadamc dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT sombuttanthitima dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT williamsgeorget dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT wannalerseboontana dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT jenkinsatobya dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT bullstevend dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels
AT jamestonyd dyedisplacementassayforsaccharidesusingbenzoxaborolehydrogels

Ejemplares similares