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Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838468/ https://www.ncbi.nlm.nih.gov/pubmed/29675275 http://dx.doi.org/10.1155/2018/9197631 |
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author | Zou, Leilei Zhu, Xiaoyu Liu, Rui Ma, Fei Yu, Manrong Liu, Hong Dai, Jinhui |
author_facet | Zou, Leilei Zhu, Xiaoyu Liu, Rui Ma, Fei Yu, Manrong Liu, Hong Dai, Jinhui |
author_sort | Zou, Leilei |
collection | PubMed |
description | PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. RESULTS: After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p < 0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p < 0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. CONCLUSIONS: Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs. |
format | Online Article Text |
id | pubmed-5838468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58384682018-04-19 Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs Zou, Leilei Zhu, Xiaoyu Liu, Rui Ma, Fei Yu, Manrong Liu, Hong Dai, Jinhui J Ophthalmol Research Article PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. RESULTS: After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p < 0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p < 0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. CONCLUSIONS: Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs. Hindawi 2018-02-20 /pmc/articles/PMC5838468/ /pubmed/29675275 http://dx.doi.org/10.1155/2018/9197631 Text en Copyright © 2018 Leilei Zou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zou, Leilei Zhu, Xiaoyu Liu, Rui Ma, Fei Yu, Manrong Liu, Hong Dai, Jinhui Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title_full | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title_fullStr | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title_full_unstemmed | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title_short | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
title_sort | effect of altered retinal cones/opsins on refractive development under monochromatic lights in guinea pigs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838468/ https://www.ncbi.nlm.nih.gov/pubmed/29675275 http://dx.doi.org/10.1155/2018/9197631 |
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