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Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs

PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and a...

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Autores principales: Zou, Leilei, Zhu, Xiaoyu, Liu, Rui, Ma, Fei, Yu, Manrong, Liu, Hong, Dai, Jinhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838468/
https://www.ncbi.nlm.nih.gov/pubmed/29675275
http://dx.doi.org/10.1155/2018/9197631
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author Zou, Leilei
Zhu, Xiaoyu
Liu, Rui
Ma, Fei
Yu, Manrong
Liu, Hong
Dai, Jinhui
author_facet Zou, Leilei
Zhu, Xiaoyu
Liu, Rui
Ma, Fei
Yu, Manrong
Liu, Hong
Dai, Jinhui
author_sort Zou, Leilei
collection PubMed
description PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. RESULTS: After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p < 0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p < 0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. CONCLUSIONS: Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs.
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spelling pubmed-58384682018-04-19 Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs Zou, Leilei Zhu, Xiaoyu Liu, Rui Ma, Fei Yu, Manrong Liu, Hong Dai, Jinhui J Ophthalmol Research Article PURPOSE: To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. METHODS: Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. RESULTS: After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p < 0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p < 0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. CONCLUSIONS: Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs. Hindawi 2018-02-20 /pmc/articles/PMC5838468/ /pubmed/29675275 http://dx.doi.org/10.1155/2018/9197631 Text en Copyright © 2018 Leilei Zou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zou, Leilei
Zhu, Xiaoyu
Liu, Rui
Ma, Fei
Yu, Manrong
Liu, Hong
Dai, Jinhui
Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title_full Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title_fullStr Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title_full_unstemmed Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title_short Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
title_sort effect of altered retinal cones/opsins on refractive development under monochromatic lights in guinea pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838468/
https://www.ncbi.nlm.nih.gov/pubmed/29675275
http://dx.doi.org/10.1155/2018/9197631
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