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Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants

BACKGROUND: A current challenge in bioprocessing is the ability to analyse critical quality attributes such as aggregation without prior purification. This study evaluated the use of fluorescent dyes (Bis‐ANS, SYPRO Orange, Thioflavin T and ProteoStat) to characterise mAb aggregates in Chinese hamst...

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Detalles Bibliográficos
Autores principales:	Oshinbolu, Sheun, Shah, Rachana, Finka, Gary, Molloy, Mike, Uden, Mark, Bracewell, Daniel G
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley & Sons, Ltd 2018
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838538/ https://www.ncbi.nlm.nih.gov/pubmed/29540956 http://dx.doi.org/10.1002/jctb.5519

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