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An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis

Exocytosis, facilitated by the exocyst, is fundamentally important for remodeling cell walls and membranes. Here, we analyzed For1F, a novel gene that encodes a fusion of an exocyst subunit (Sec10) and an actin nucleation factor (formin). We showed that the fusion occurred early in moss evolution an...

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Autores principales: van Gisbergen, Peter A.C., Wu, Shu-Zon, Chang, Mingqin, Pattavina, Kelli A., Bartlett, Madelaine E., Bezanilla, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839782/
https://www.ncbi.nlm.nih.gov/pubmed/29374070
http://dx.doi.org/10.1083/jcb.201705084
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author van Gisbergen, Peter A.C.
Wu, Shu-Zon
Chang, Mingqin
Pattavina, Kelli A.
Bartlett, Madelaine E.
Bezanilla, Magdalena
author_facet van Gisbergen, Peter A.C.
Wu, Shu-Zon
Chang, Mingqin
Pattavina, Kelli A.
Bartlett, Madelaine E.
Bezanilla, Magdalena
author_sort van Gisbergen, Peter A.C.
collection PubMed
description Exocytosis, facilitated by the exocyst, is fundamentally important for remodeling cell walls and membranes. Here, we analyzed For1F, a novel gene that encodes a fusion of an exocyst subunit (Sec10) and an actin nucleation factor (formin). We showed that the fusion occurred early in moss evolution and has been retained for more than 170 million years. In Physcomitrella patens, For1F is essential, and the expressed protein is a fusion of Sec10 and formin. Reduction of For1F or actin filaments inhibits exocytosis, and For1F dynamically associates with Sec6, another exocyst subunit, in an actin-dependent manner. Complementation experiments demonstrate that constitutive expression of either half of the gene or the paralogous Sec10b rescues loss of For1F, suggesting that fusion of the two domains is not essential, consistent with findings in yeast, where formin and the exocyst are linked noncovalently. Although not essential, the fusion may have had selective advantages and provides a unique opportunity to probe actin regulation of exocytosis.
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spelling pubmed-58397822018-09-05 An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis van Gisbergen, Peter A.C. Wu, Shu-Zon Chang, Mingqin Pattavina, Kelli A. Bartlett, Madelaine E. Bezanilla, Magdalena J Cell Biol Research Articles Exocytosis, facilitated by the exocyst, is fundamentally important for remodeling cell walls and membranes. Here, we analyzed For1F, a novel gene that encodes a fusion of an exocyst subunit (Sec10) and an actin nucleation factor (formin). We showed that the fusion occurred early in moss evolution and has been retained for more than 170 million years. In Physcomitrella patens, For1F is essential, and the expressed protein is a fusion of Sec10 and formin. Reduction of For1F or actin filaments inhibits exocytosis, and For1F dynamically associates with Sec6, another exocyst subunit, in an actin-dependent manner. Complementation experiments demonstrate that constitutive expression of either half of the gene or the paralogous Sec10b rescues loss of For1F, suggesting that fusion of the two domains is not essential, consistent with findings in yeast, where formin and the exocyst are linked noncovalently. Although not essential, the fusion may have had selective advantages and provides a unique opportunity to probe actin regulation of exocytosis. Rockefeller University Press 2018-03-05 /pmc/articles/PMC5839782/ /pubmed/29374070 http://dx.doi.org/10.1083/jcb.201705084 Text en © 2018 van Gisbergen et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
van Gisbergen, Peter A.C.
Wu, Shu-Zon
Chang, Mingqin
Pattavina, Kelli A.
Bartlett, Madelaine E.
Bezanilla, Magdalena
An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title_full An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title_fullStr An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title_full_unstemmed An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title_short An ancient Sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
title_sort ancient sec10–formin fusion provides insights into actin-mediated regulation of exocytosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839782/
https://www.ncbi.nlm.nih.gov/pubmed/29374070
http://dx.doi.org/10.1083/jcb.201705084
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