Open-source recombinant monoclonal secondary nanobodies

Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies...

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Detalles Bibliográficos
Autor principal: Ewers, Helge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Commentaries
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839800/
https://www.ncbi.nlm.nih.gov/pubmed/29444803
http://dx.doi.org/10.1083/jcb.201802025
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author Ewers, Helge
author_facet Ewers, Helge
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description Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.
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spelling pubmed-58398002018-09-05 Open-source recombinant monoclonal secondary nanobodies Ewers, Helge J Cell Biol Commentaries Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays. Rockefeller University Press 2018-03-05 /pmc/articles/PMC5839800/ /pubmed/29444803 http://dx.doi.org/10.1083/jcb.201802025 Text en © 2018 Ewers http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Commentaries
Ewers, Helge
Open-source recombinant monoclonal secondary nanobodies
title Open-source recombinant monoclonal secondary nanobodies
title_full Open-source recombinant monoclonal secondary nanobodies
title_fullStr Open-source recombinant monoclonal secondary nanobodies
title_full_unstemmed Open-source recombinant monoclonal secondary nanobodies
title_short Open-source recombinant monoclonal secondary nanobodies
title_sort open-source recombinant monoclonal secondary nanobodies
topic Commentaries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839800/
https://www.ncbi.nlm.nih.gov/pubmed/29444803
http://dx.doi.org/10.1083/jcb.201802025
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