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Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments

In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrug resistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype...

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Autores principales: Chen, Xiu P., Ali, Liaqat, Wu, Li-Yun, Liu, Can, Gang, Chen X., Huang, Qi F., Ruan, Jing H., Bao, Song Y., Rao, Yun P., Yu, DaoJin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840168/
https://www.ncbi.nlm.nih.gov/pubmed/29552003
http://dx.doi.org/10.3389/fmicb.2018.00367
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author Chen, Xiu P.
Ali, Liaqat
Wu, Li-Yun
Liu, Can
Gang, Chen X.
Huang, Qi F.
Ruan, Jing H.
Bao, Song Y.
Rao, Yun P.
Yu, DaoJin
author_facet Chen, Xiu P.
Ali, Liaqat
Wu, Li-Yun
Liu, Can
Gang, Chen X.
Huang, Qi F.
Ruan, Jing H.
Bao, Song Y.
Rao, Yun P.
Yu, DaoJin
author_sort Chen, Xiu P.
collection PubMed
description In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrug resistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype. Different concentrations of N and P were added to make one control group and four treatment groups. The glass tube method was used to determine biofilm-forming capabilities. Real-time PCR was used to detect the mRNA abundance of six biofilm-formation-related genes in E. coli. No resistant strains were isolated from the control group; meanwhile, multidrug resistance rates were high in the treatment groups. Expression of the biofilm-associated genes luxS, flhD, fliA, motA, and fimH was detected in all treatment groups; however, there was no expression of mqsR. The expression of luxS, flhD, fliA, motA, and fimH significantly correlated with the concentration of N and P, as well as with the appearance and duration of multidrug resistance in different groups. Overall, the results of this study suggest that biofilm-forming ability plays a key role in the formation of multidrug resistance in E. coli after the addition of N and P to a microcosm.
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spelling pubmed-58401682018-03-16 Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments Chen, Xiu P. Ali, Liaqat Wu, Li-Yun Liu, Can Gang, Chen X. Huang, Qi F. Ruan, Jing H. Bao, Song Y. Rao, Yun P. Yu, DaoJin Front Microbiol Microbiology In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrug resistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype. Different concentrations of N and P were added to make one control group and four treatment groups. The glass tube method was used to determine biofilm-forming capabilities. Real-time PCR was used to detect the mRNA abundance of six biofilm-formation-related genes in E. coli. No resistant strains were isolated from the control group; meanwhile, multidrug resistance rates were high in the treatment groups. Expression of the biofilm-associated genes luxS, flhD, fliA, motA, and fimH was detected in all treatment groups; however, there was no expression of mqsR. The expression of luxS, flhD, fliA, motA, and fimH significantly correlated with the concentration of N and P, as well as with the appearance and duration of multidrug resistance in different groups. Overall, the results of this study suggest that biofilm-forming ability plays a key role in the formation of multidrug resistance in E. coli after the addition of N and P to a microcosm. Frontiers Media S.A. 2018-03-02 /pmc/articles/PMC5840168/ /pubmed/29552003 http://dx.doi.org/10.3389/fmicb.2018.00367 Text en Copyright © 2018 Chen, Ali, Wu, Liu, Gang, Huang, Ruan, Bao, Rao and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chen, Xiu P.
Ali, Liaqat
Wu, Li-Yun
Liu, Can
Gang, Chen X.
Huang, Qi F.
Ruan, Jing H.
Bao, Song Y.
Rao, Yun P.
Yu, DaoJin
Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title_full Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title_fullStr Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title_full_unstemmed Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title_short Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments
title_sort biofilm formation plays a role in the formation of multidrug-resistant escherichia coli toward nutrients in microcosm experiments
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840168/
https://www.ncbi.nlm.nih.gov/pubmed/29552003
http://dx.doi.org/10.3389/fmicb.2018.00367
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