Cargando…
Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors
Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. In the rubber tree, this fungus causes the Corynespora leaf fall (CLF) disease, which increa...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840194/ https://www.ncbi.nlm.nih.gov/pubmed/29551995 http://dx.doi.org/10.3389/fmicb.2018.00276 |
_version_ | 1783304526625767424 |
---|---|
author | Lopez, David Ribeiro, Sébastien Label, Philippe Fumanal, Boris Venisse, Jean-Stéphane Kohler, Annegret de Oliveira, Ricardo R. Labutti, Kurt Lipzen, Anna Lail, Kathleen Bauer, Diane Ohm, Robin A. Barry, Kerrie W. Spatafora, Joseph Grigoriev, Igor V. Martin, Francis M. Pujade-Renaud, Valérie |
author_facet | Lopez, David Ribeiro, Sébastien Label, Philippe Fumanal, Boris Venisse, Jean-Stéphane Kohler, Annegret de Oliveira, Ricardo R. Labutti, Kurt Lipzen, Anna Lail, Kathleen Bauer, Diane Ohm, Robin A. Barry, Kerrie W. Spatafora, Joseph Grigoriev, Igor V. Martin, Francis M. Pujade-Renaud, Valérie |
author_sort | Lopez, David |
collection | PubMed |
description | Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. In the rubber tree, this fungus causes the Corynespora leaf fall (CLF) disease, which increasingly affects natural rubber production in Asia and Africa. It has also been found as an endophyte in South American rubber plantations where no CLF outbreak has yet occurred. The C. cassiicola species is genetically highly diverse, but no clear relationship has been evidenced between phylogenetic lineage and pathogenicity. Cassiicolin, a small glycosylated secreted protein effector, is thought to be involved in the necrotrophic interaction with the rubber tree but some virulent C. cassiicola isolates do not have a cassiicolin gene. This study set out to identify other putative effectors involved in CLF. The genome of a highly virulent C. cassiicola isolate from the rubber tree (CCP) was sequenced and assembled. In silico prediction revealed 2870 putative effectors, comprising CAZymes, lipases, peptidases, secreted proteins and enzymes associated with secondary metabolism. Comparison with the genomes of 44 other fungal species, focusing on effector content, revealed a striking proximity with phylogenetically unrelated species (Colletotrichum acutatum, Colletotrichum gloesporioides, Fusarium oxysporum, nectria hematococca, and Botrosphaeria dothidea) sharing life style plasticity and broad host range. Candidate effectors involved in the compatible interaction with the rubber tree were identified by transcriptomic analysis. Differentially expressed genes included 92 putative effectors, among which cassiicolin and two other secreted singleton proteins. Finally, the genomes of 35 C. cassiicola isolates representing the genetic diversity of the species were sequenced and assembled, and putative effectors identified. At the intraspecific level, effector-based classification was found to be highly consistent with the phylogenomic trees. Identification of lineage-specific effectors is a key step toward understanding C. cassiicola virulence and host specialization mechanisms. |
format | Online Article Text |
id | pubmed-5840194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58401942018-03-16 Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors Lopez, David Ribeiro, Sébastien Label, Philippe Fumanal, Boris Venisse, Jean-Stéphane Kohler, Annegret de Oliveira, Ricardo R. Labutti, Kurt Lipzen, Anna Lail, Kathleen Bauer, Diane Ohm, Robin A. Barry, Kerrie W. Spatafora, Joseph Grigoriev, Igor V. Martin, Francis M. Pujade-Renaud, Valérie Front Microbiol Microbiology Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. In the rubber tree, this fungus causes the Corynespora leaf fall (CLF) disease, which increasingly affects natural rubber production in Asia and Africa. It has also been found as an endophyte in South American rubber plantations where no CLF outbreak has yet occurred. The C. cassiicola species is genetically highly diverse, but no clear relationship has been evidenced between phylogenetic lineage and pathogenicity. Cassiicolin, a small glycosylated secreted protein effector, is thought to be involved in the necrotrophic interaction with the rubber tree but some virulent C. cassiicola isolates do not have a cassiicolin gene. This study set out to identify other putative effectors involved in CLF. The genome of a highly virulent C. cassiicola isolate from the rubber tree (CCP) was sequenced and assembled. In silico prediction revealed 2870 putative effectors, comprising CAZymes, lipases, peptidases, secreted proteins and enzymes associated with secondary metabolism. Comparison with the genomes of 44 other fungal species, focusing on effector content, revealed a striking proximity with phylogenetically unrelated species (Colletotrichum acutatum, Colletotrichum gloesporioides, Fusarium oxysporum, nectria hematococca, and Botrosphaeria dothidea) sharing life style plasticity and broad host range. Candidate effectors involved in the compatible interaction with the rubber tree were identified by transcriptomic analysis. Differentially expressed genes included 92 putative effectors, among which cassiicolin and two other secreted singleton proteins. Finally, the genomes of 35 C. cassiicola isolates representing the genetic diversity of the species were sequenced and assembled, and putative effectors identified. At the intraspecific level, effector-based classification was found to be highly consistent with the phylogenomic trees. Identification of lineage-specific effectors is a key step toward understanding C. cassiicola virulence and host specialization mechanisms. Frontiers Media S.A. 2018-03-02 /pmc/articles/PMC5840194/ /pubmed/29551995 http://dx.doi.org/10.3389/fmicb.2018.00276 Text en Copyright © 2018 Lopez, Ribeiro, Label, Fumanal, Venisse, Kohler, de Oliveira, Labutti, Lipzen, Lail, Bauer, Ohm, Barry, Spatafora, Grigoriev, Martin and Pujade-Renaud. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lopez, David Ribeiro, Sébastien Label, Philippe Fumanal, Boris Venisse, Jean-Stéphane Kohler, Annegret de Oliveira, Ricardo R. Labutti, Kurt Lipzen, Anna Lail, Kathleen Bauer, Diane Ohm, Robin A. Barry, Kerrie W. Spatafora, Joseph Grigoriev, Igor V. Martin, Francis M. Pujade-Renaud, Valérie Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title | Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title_full | Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title_fullStr | Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title_full_unstemmed | Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title_short | Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors |
title_sort | genome-wide analysis of corynespora cassiicola leaf fall disease putative effectors |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840194/ https://www.ncbi.nlm.nih.gov/pubmed/29551995 http://dx.doi.org/10.3389/fmicb.2018.00276 |
work_keys_str_mv | AT lopezdavid genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT ribeirosebastien genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT labelphilippe genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT fumanalboris genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT venissejeanstephane genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT kohlerannegret genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT deoliveiraricardor genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT labuttikurt genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT lipzenanna genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT lailkathleen genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT bauerdiane genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT ohmrobina genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT barrykerriew genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT spataforajoseph genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT grigorievigorv genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT martinfrancism genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors AT pujaderenaudvalerie genomewideanalysisofcorynesporacassiicolaleaffalldiseaseputativeeffectors |