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Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection

Environmental bacteria of the genus Legionella naturally parasitize free-living amoebae. Upon inhalation of bacteria-laden aerosols, the opportunistic pathogens grow intracellularly in alveolar macrophages and can cause a life-threatening pneumonia termed Legionnaires' disease. Intracellular re...

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Autores principales: Swart, A. Leoni, Harrison, Christopher F., Eichinger, Ludwig, Steinert, Michael, Hilbi, Hubert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840211/
https://www.ncbi.nlm.nih.gov/pubmed/29552544
http://dx.doi.org/10.3389/fcimb.2018.00061
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author Swart, A. Leoni
Harrison, Christopher F.
Eichinger, Ludwig
Steinert, Michael
Hilbi, Hubert
author_facet Swart, A. Leoni
Harrison, Christopher F.
Eichinger, Ludwig
Steinert, Michael
Hilbi, Hubert
author_sort Swart, A. Leoni
collection PubMed
description Environmental bacteria of the genus Legionella naturally parasitize free-living amoebae. Upon inhalation of bacteria-laden aerosols, the opportunistic pathogens grow intracellularly in alveolar macrophages and can cause a life-threatening pneumonia termed Legionnaires' disease. Intracellular replication in amoebae and macrophages takes place in a unique membrane-bound compartment, the Legionella-containing vacuole (LCV). LCV formation requires the bacterial Icm/Dot type IV secretion system, which translocates literally hundreds of “effector” proteins into host cells, where they modulate crucial cellular processes for the pathogen's benefit. The mechanism of LCV formation appears to be evolutionarily conserved, and therefore, amoebae are not only ecologically significant niches for Legionella spp., but also useful cellular models for eukaryotic phagocytes. In particular, Acanthamoeba castellanii and Dictyostelium discoideum emerged over the last years as versatile and powerful models. Using genetic, biochemical and cell biological approaches, molecular interactions between amoebae and Legionella pneumophila have recently been investigated in detail with a focus on the role of phosphoinositide lipids, small and large GTPases, autophagy components and the retromer complex, as well as on bacterial effectors targeting these host factors.
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spelling pubmed-58402112018-03-16 Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection Swart, A. Leoni Harrison, Christopher F. Eichinger, Ludwig Steinert, Michael Hilbi, Hubert Front Cell Infect Microbiol Microbiology Environmental bacteria of the genus Legionella naturally parasitize free-living amoebae. Upon inhalation of bacteria-laden aerosols, the opportunistic pathogens grow intracellularly in alveolar macrophages and can cause a life-threatening pneumonia termed Legionnaires' disease. Intracellular replication in amoebae and macrophages takes place in a unique membrane-bound compartment, the Legionella-containing vacuole (LCV). LCV formation requires the bacterial Icm/Dot type IV secretion system, which translocates literally hundreds of “effector” proteins into host cells, where they modulate crucial cellular processes for the pathogen's benefit. The mechanism of LCV formation appears to be evolutionarily conserved, and therefore, amoebae are not only ecologically significant niches for Legionella spp., but also useful cellular models for eukaryotic phagocytes. In particular, Acanthamoeba castellanii and Dictyostelium discoideum emerged over the last years as versatile and powerful models. Using genetic, biochemical and cell biological approaches, molecular interactions between amoebae and Legionella pneumophila have recently been investigated in detail with a focus on the role of phosphoinositide lipids, small and large GTPases, autophagy components and the retromer complex, as well as on bacterial effectors targeting these host factors. Frontiers Media S.A. 2018-03-02 /pmc/articles/PMC5840211/ /pubmed/29552544 http://dx.doi.org/10.3389/fcimb.2018.00061 Text en Copyright © 2018 Swart, Harrison, Eichinger, Steinert and Hilbi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Swart, A. Leoni
Harrison, Christopher F.
Eichinger, Ludwig
Steinert, Michael
Hilbi, Hubert
Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title_full Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title_fullStr Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title_full_unstemmed Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title_short Acanthamoeba and Dictyostelium as Cellular Models for Legionella Infection
title_sort acanthamoeba and dictyostelium as cellular models for legionella infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840211/
https://www.ncbi.nlm.nih.gov/pubmed/29552544
http://dx.doi.org/10.3389/fcimb.2018.00061
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