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Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress

Cytoskeleton plays a vital role in stress tolerance; however, involvement of intermediate filaments (IFs) in such a response remains elusive in crop plants. This study provides clear evidence about the unique involvement of IFs in cellular protection against abiotic stress in rice. Transcript abunda...

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Autores principales: Soda, Neelam, Gupta, Brijesh K., Anwar, Khalid, Sharan, Ashutosh, Govindjee, Singla-Pareek, Sneh L., Pareek, Ashwani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840354/
https://www.ncbi.nlm.nih.gov/pubmed/29511223
http://dx.doi.org/10.1038/s41598-018-22131-0
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author Soda, Neelam
Gupta, Brijesh K.
Anwar, Khalid
Sharan, Ashutosh
Govindjee
Singla-Pareek, Sneh L.
Pareek, Ashwani
author_facet Soda, Neelam
Gupta, Brijesh K.
Anwar, Khalid
Sharan, Ashutosh
Govindjee
Singla-Pareek, Sneh L.
Pareek, Ashwani
author_sort Soda, Neelam
collection PubMed
description Cytoskeleton plays a vital role in stress tolerance; however, involvement of intermediate filaments (IFs) in such a response remains elusive in crop plants. This study provides clear evidence about the unique involvement of IFs in cellular protection against abiotic stress in rice. Transcript abundance of Oryza sativa intermediate filament (OsIF) encoding gene showed 2–10 fold up-regulation under different abiotic stress. Overexpression of OsIF in transgenic rice enhanced tolerance to salinity and heat stress, while its knock-down (KD) rendered plants more sensitive thereby indicating the role of IFs in promoting survival under stress. Seeds of OsIF overexpression rice germinated normally in the presence of high salt, showed better growth, maintained chloroplast ultrastructure and favourable K(+)/Na(+) ratio than the wild type (WT) and KD plants. Analysis of photosynthesis and chlorophyll a fluorescence data suggested better performance of both photosystem I and II in the OsIF overexpression rice under salinity stress as compared to the WT and KD. Under salinity and high temperature stress, OsIF overexpressing plants could maintain significantly high yield, while the WT and KD plants could not. Further, metabolite profiling revealed a 2–4 fold higher accumulation of proline and trehalose in OsIF overexpressing rice than WT, under salinity stress.
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spelling pubmed-58403542018-03-13 Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress Soda, Neelam Gupta, Brijesh K. Anwar, Khalid Sharan, Ashutosh Govindjee Singla-Pareek, Sneh L. Pareek, Ashwani Sci Rep Article Cytoskeleton plays a vital role in stress tolerance; however, involvement of intermediate filaments (IFs) in such a response remains elusive in crop plants. This study provides clear evidence about the unique involvement of IFs in cellular protection against abiotic stress in rice. Transcript abundance of Oryza sativa intermediate filament (OsIF) encoding gene showed 2–10 fold up-regulation under different abiotic stress. Overexpression of OsIF in transgenic rice enhanced tolerance to salinity and heat stress, while its knock-down (KD) rendered plants more sensitive thereby indicating the role of IFs in promoting survival under stress. Seeds of OsIF overexpression rice germinated normally in the presence of high salt, showed better growth, maintained chloroplast ultrastructure and favourable K(+)/Na(+) ratio than the wild type (WT) and KD plants. Analysis of photosynthesis and chlorophyll a fluorescence data suggested better performance of both photosystem I and II in the OsIF overexpression rice under salinity stress as compared to the WT and KD. Under salinity and high temperature stress, OsIF overexpressing plants could maintain significantly high yield, while the WT and KD plants could not. Further, metabolite profiling revealed a 2–4 fold higher accumulation of proline and trehalose in OsIF overexpressing rice than WT, under salinity stress. Nature Publishing Group UK 2018-03-06 /pmc/articles/PMC5840354/ /pubmed/29511223 http://dx.doi.org/10.1038/s41598-018-22131-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Soda, Neelam
Gupta, Brijesh K.
Anwar, Khalid
Sharan, Ashutosh
Govindjee
Singla-Pareek, Sneh L.
Pareek, Ashwani
Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title_full Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title_fullStr Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title_full_unstemmed Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title_short Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress
title_sort rice intermediate filament, osif, stabilizes photosynthetic machinery and yield under salinity and heat stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840354/
https://www.ncbi.nlm.nih.gov/pubmed/29511223
http://dx.doi.org/10.1038/s41598-018-22131-0
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