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Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites
The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840753/ https://www.ncbi.nlm.nih.gov/pubmed/29552124 http://dx.doi.org/10.3892/ol.2018.7995 |
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author | Mytar, Bożenna Stec, Małgorzata Szatanek, Rafał Węglarczyk, Kazimierz Szewczyk, Katarzyna Szczepanik, Antoni Drabik, Grażyna Baran, Jarek Siedlar, Maciej Baj-Krzyworzeka, Monika |
author_facet | Mytar, Bożenna Stec, Małgorzata Szatanek, Rafał Węglarczyk, Kazimierz Szewczyk, Katarzyna Szczepanik, Antoni Drabik, Grażyna Baran, Jarek Siedlar, Maciej Baj-Krzyworzeka, Monika |
author_sort | Mytar, Bożenna |
collection | PubMed |
description | The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. All cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum. These cell lines were grown as an adherent monolayer with doubling time ranging between 25 h (GC1436 cell line) and 30–34 h (GC1401 and GC1415, respectively). All cells showed morphological features of epithelial-like cells, forming sheets of polygonal cells. Chromosomal analysis showed that the modal numbers ranged from 52 (GC1401), 51–56 (GC1415) and 106 (GC1436). High heterogeneity, resulting from several structural and numerical chromosomal abnormalities were evident in all cell lines. The surface marker expression suggested a tumor origin of the cells, and indicated the intestinal phenotype of a GC (CD10(+), MUC1). All three cell lines were tumorigenic but not metastatic, in vivo, in NOD/SCID mice. The lack of metastatic potential was suggested by the lack of aldehyde dehydrogenase 1A1 activity. In conclusion, these newly established GC cell lines widen the feasibility of the functional studies on biology of GC as well as drug testing for potential therapeutic purposes. |
format | Online Article Text |
id | pubmed-5840753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58407532018-03-16 Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites Mytar, Bożenna Stec, Małgorzata Szatanek, Rafał Węglarczyk, Kazimierz Szewczyk, Katarzyna Szczepanik, Antoni Drabik, Grażyna Baran, Jarek Siedlar, Maciej Baj-Krzyworzeka, Monika Oncol Lett Articles The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. All cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum. These cell lines were grown as an adherent monolayer with doubling time ranging between 25 h (GC1436 cell line) and 30–34 h (GC1401 and GC1415, respectively). All cells showed morphological features of epithelial-like cells, forming sheets of polygonal cells. Chromosomal analysis showed that the modal numbers ranged from 52 (GC1401), 51–56 (GC1415) and 106 (GC1436). High heterogeneity, resulting from several structural and numerical chromosomal abnormalities were evident in all cell lines. The surface marker expression suggested a tumor origin of the cells, and indicated the intestinal phenotype of a GC (CD10(+), MUC1). All three cell lines were tumorigenic but not metastatic, in vivo, in NOD/SCID mice. The lack of metastatic potential was suggested by the lack of aldehyde dehydrogenase 1A1 activity. In conclusion, these newly established GC cell lines widen the feasibility of the functional studies on biology of GC as well as drug testing for potential therapeutic purposes. D.A. Spandidos 2018-04 2018-02-08 /pmc/articles/PMC5840753/ /pubmed/29552124 http://dx.doi.org/10.3892/ol.2018.7995 Text en Copyright: © Mytar et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Mytar, Bożenna Stec, Małgorzata Szatanek, Rafał Węglarczyk, Kazimierz Szewczyk, Katarzyna Szczepanik, Antoni Drabik, Grażyna Baran, Jarek Siedlar, Maciej Baj-Krzyworzeka, Monika Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title | Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title_full | Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title_fullStr | Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title_full_unstemmed | Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title_short | Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
title_sort | characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840753/ https://www.ncbi.nlm.nih.gov/pubmed/29552124 http://dx.doi.org/10.3892/ol.2018.7995 |
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