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MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment

Anesthesia-induced cognitive impairment is a recognized clinical phenomenon. The present study aimed to investigate the effect of microRNA-383 (miR-383) expression on propofol-induced learning and memory impairment. In total, 48 male Sprague-Dawley rats (weight, 250±10 g) were randomly divided into...

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Autores principales: Wang, Xinlei, Ding, Guoyou, Lai, Wei, Liu, Shiwen, Shuai, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840935/
https://www.ncbi.nlm.nih.gov/pubmed/29545833
http://dx.doi.org/10.3892/etm.2018.5838
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author Wang, Xinlei
Ding, Guoyou
Lai, Wei
Liu, Shiwen
Shuai, Jun
author_facet Wang, Xinlei
Ding, Guoyou
Lai, Wei
Liu, Shiwen
Shuai, Jun
author_sort Wang, Xinlei
collection PubMed
description Anesthesia-induced cognitive impairment is a recognized clinical phenomenon. The present study aimed to investigate the effect of microRNA-383 (miR-383) expression on propofol-induced learning and memory impairment. In total, 48 male Sprague-Dawley rats (weight, 250±10 g) were randomly divided into four groups (n=12 each): Control group, and three groups of rats that were anesthetized with propofol for 6 h and untreated (propofol model group), treated with a constructed lentivirus vector expressing miR-383 mimics (mimic + propofol group), or treated with miR-383 scramble (scramble + propofol group). The learning memory ability, hippocampal neuron apoptosis and expression of apoptosis-associated factors were detected using reverse transcription-quantitiative polymerase chain reaction and western blot analysis. Propofol treatment significantly reduced the relative mRNA and protein expression of miR-383, induced neuron apoptosis, upregulated the Bax/Bcl-2 ratio, downregulated the relative mRNA and protein expression levels of postsynaptic density protein 95 and cAMP-response element binding protein, and inactivated the phosphoinositide 3-kinase/protein kinase B signaling pathway. By contrast, miR-383 mimics significantly altered the propofol-induced dysregulation of the aforementioned factors. In conclusion, miR-383 mimic was able to repair propofol-induced cognitive impairment via protecting against hippocampal neuron apoptosis and dysregulation of related factors. The present study suggested that miR-383 may be used as a potential therapeutic target for the clinical treatment of cognitive impairment induced by propofol anesthesia.
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spelling pubmed-58409352018-03-15 MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment Wang, Xinlei Ding, Guoyou Lai, Wei Liu, Shiwen Shuai, Jun Exp Ther Med Articles Anesthesia-induced cognitive impairment is a recognized clinical phenomenon. The present study aimed to investigate the effect of microRNA-383 (miR-383) expression on propofol-induced learning and memory impairment. In total, 48 male Sprague-Dawley rats (weight, 250±10 g) were randomly divided into four groups (n=12 each): Control group, and three groups of rats that were anesthetized with propofol for 6 h and untreated (propofol model group), treated with a constructed lentivirus vector expressing miR-383 mimics (mimic + propofol group), or treated with miR-383 scramble (scramble + propofol group). The learning memory ability, hippocampal neuron apoptosis and expression of apoptosis-associated factors were detected using reverse transcription-quantitiative polymerase chain reaction and western blot analysis. Propofol treatment significantly reduced the relative mRNA and protein expression of miR-383, induced neuron apoptosis, upregulated the Bax/Bcl-2 ratio, downregulated the relative mRNA and protein expression levels of postsynaptic density protein 95 and cAMP-response element binding protein, and inactivated the phosphoinositide 3-kinase/protein kinase B signaling pathway. By contrast, miR-383 mimics significantly altered the propofol-induced dysregulation of the aforementioned factors. In conclusion, miR-383 mimic was able to repair propofol-induced cognitive impairment via protecting against hippocampal neuron apoptosis and dysregulation of related factors. The present study suggested that miR-383 may be used as a potential therapeutic target for the clinical treatment of cognitive impairment induced by propofol anesthesia. D.A. Spandidos 2018-04 2018-02-05 /pmc/articles/PMC5840935/ /pubmed/29545833 http://dx.doi.org/10.3892/etm.2018.5838 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Xinlei
Ding, Guoyou
Lai, Wei
Liu, Shiwen
Shuai, Jun
MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title_full MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title_fullStr MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title_full_unstemmed MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title_short MicroRNA-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
title_sort microrna-383 upregulation protects against propofol-induced hippocampal neuron apoptosis and cognitive impairment
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840935/
https://www.ncbi.nlm.nih.gov/pubmed/29545833
http://dx.doi.org/10.3892/etm.2018.5838
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