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Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb
Non-invasive methods to track the progress of stem cell therapies are important in the development of future regenerative therapies. Super-paramagnetic iron oxide particles (SPIOs) have previously been applied to track cells using magnetic resonance imaging (MRI) in vivo in non-regenerative animal m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840951/ https://www.ncbi.nlm.nih.gov/pubmed/29545849 http://dx.doi.org/10.3892/etm.2018.5865 |
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author | Lauridsen, Henrik Foldager, Casper Bindzus Hansen, Line Pedersen, Michael |
author_facet | Lauridsen, Henrik Foldager, Casper Bindzus Hansen, Line Pedersen, Michael |
author_sort | Lauridsen, Henrik |
collection | PubMed |
description | Non-invasive methods to track the progress of stem cell therapies are important in the development of future regenerative therapies. Super-paramagnetic iron oxide particles (SPIOs) have previously been applied to track cells using magnetic resonance imaging (MRI) in vivo in non-regenerative animal models. To the best of the author's knowledge, the present study investigated for the first time, the feasibility of tracking SPIO labeled cells in an intrinsic regenerative environment, the regenerating limb of the axolotl, and investigated the homing of stem cell-like blastema cells to the regenerative zone. Viability and labeling success of labeled axolotl blastema cells was tested in vitro using cell culture and histology. SPIO labeling was performed in situ by intramuscular injections and mapped using MRI. Enhanced permeability and retention (EPR) effects were evaluated in the blastema, liver, heart, kidney and a back muscle. Finally, SPIO/Fluorophore-labeled blastema cells were injected intravascularly and tracked using MRI and fluorescence imaging. It was demonstrated that SPIO labeling had no effect on axolotl cell viability in vitro. In situ labeling resulted in an MRI signal alteration during 48 days of regeneration. EPR effect of unbound SPIO was observed only in the liver. MRI tracking revealed increased concentrations of SPIO labeled blastema cells in the liver, kidney and heart, however not the blastema of intravascularly injected axolotls. In conclusion, the results demonstrated that SPIO labeling facilitated non-invasive tracking of injected cells in the regenerating axolotl limb. An early homing mechanism of injected blastema cells to an injury site was not observed. |
format | Online Article Text |
id | pubmed-5840951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58409512018-03-15 Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb Lauridsen, Henrik Foldager, Casper Bindzus Hansen, Line Pedersen, Michael Exp Ther Med Articles Non-invasive methods to track the progress of stem cell therapies are important in the development of future regenerative therapies. Super-paramagnetic iron oxide particles (SPIOs) have previously been applied to track cells using magnetic resonance imaging (MRI) in vivo in non-regenerative animal models. To the best of the author's knowledge, the present study investigated for the first time, the feasibility of tracking SPIO labeled cells in an intrinsic regenerative environment, the regenerating limb of the axolotl, and investigated the homing of stem cell-like blastema cells to the regenerative zone. Viability and labeling success of labeled axolotl blastema cells was tested in vitro using cell culture and histology. SPIO labeling was performed in situ by intramuscular injections and mapped using MRI. Enhanced permeability and retention (EPR) effects were evaluated in the blastema, liver, heart, kidney and a back muscle. Finally, SPIO/Fluorophore-labeled blastema cells were injected intravascularly and tracked using MRI and fluorescence imaging. It was demonstrated that SPIO labeling had no effect on axolotl cell viability in vitro. In situ labeling resulted in an MRI signal alteration during 48 days of regeneration. EPR effect of unbound SPIO was observed only in the liver. MRI tracking revealed increased concentrations of SPIO labeled blastema cells in the liver, kidney and heart, however not the blastema of intravascularly injected axolotls. In conclusion, the results demonstrated that SPIO labeling facilitated non-invasive tracking of injected cells in the regenerating axolotl limb. An early homing mechanism of injected blastema cells to an injury site was not observed. D.A. Spandidos 2018-04 2018-02-14 /pmc/articles/PMC5840951/ /pubmed/29545849 http://dx.doi.org/10.3892/etm.2018.5865 Text en Copyright: © Lauridsen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lauridsen, Henrik Foldager, Casper Bindzus Hansen, Line Pedersen, Michael Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title | Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title_full | Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title_fullStr | Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title_full_unstemmed | Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title_short | Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb |
title_sort | non-invasive cell tracking of spio labeled cells in an intrinsic regenerative environment: the axolotl limb |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5840951/ https://www.ncbi.nlm.nih.gov/pubmed/29545849 http://dx.doi.org/10.3892/etm.2018.5865 |
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