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Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV iso...

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Detalles Bibliográficos
Autores principales: Wu, Xiangju, Qi, Jing, Cong, Xiaoyan, Chen, Lei, Hu, Yue, Yoo, Dongwan, Wang, Guisheng, Tian, Fulin, Li, Feng, Sun, Wenbo, Chen, Zhi, Guo, Lihui, Wu, Jiaqiang, Li, Jun, Wang, Jinbao, Zhao, Xiaomin, Du, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841097/
https://www.ncbi.nlm.nih.gov/pubmed/29682543
http://dx.doi.org/10.1155/2018/4315861
Descripción
Sumario:Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.