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Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV iso...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841097/ https://www.ncbi.nlm.nih.gov/pubmed/29682543 http://dx.doi.org/10.1155/2018/4315861 |
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author | Wu, Xiangju Qi, Jing Cong, Xiaoyan Chen, Lei Hu, Yue Yoo, Dongwan Wang, Guisheng Tian, Fulin Li, Feng Sun, Wenbo Chen, Zhi Guo, Lihui Wu, Jiaqiang Li, Jun Wang, Jinbao Zhao, Xiaomin Du, Yijun |
author_facet | Wu, Xiangju Qi, Jing Cong, Xiaoyan Chen, Lei Hu, Yue Yoo, Dongwan Wang, Guisheng Tian, Fulin Li, Feng Sun, Wenbo Chen, Zhi Guo, Lihui Wu, Jiaqiang Li, Jun Wang, Jinbao Zhao, Xiaomin Du, Yijun |
author_sort | Wu, Xiangju |
collection | PubMed |
description | Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV. |
format | Online Article Text |
id | pubmed-5841097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58410972018-04-21 Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line Wu, Xiangju Qi, Jing Cong, Xiaoyan Chen, Lei Hu, Yue Yoo, Dongwan Wang, Guisheng Tian, Fulin Li, Feng Sun, Wenbo Chen, Zhi Guo, Lihui Wu, Jiaqiang Li, Jun Wang, Jinbao Zhao, Xiaomin Du, Yijun Biomed Res Int Research Article Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV. Hindawi 2018-02-21 /pmc/articles/PMC5841097/ /pubmed/29682543 http://dx.doi.org/10.1155/2018/4315861 Text en Copyright © 2018 Xiangju Wu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Xiangju Qi, Jing Cong, Xiaoyan Chen, Lei Hu, Yue Yoo, Dongwan Wang, Guisheng Tian, Fulin Li, Feng Sun, Wenbo Chen, Zhi Guo, Lihui Wu, Jiaqiang Li, Jun Wang, Jinbao Zhao, Xiaomin Du, Yijun Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title | Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title_full | Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title_fullStr | Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title_full_unstemmed | Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title_short | Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line |
title_sort | establishment and characterization of a high and stable porcine cd163-expressing marc-145 cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841097/ https://www.ncbi.nlm.nih.gov/pubmed/29682543 http://dx.doi.org/10.1155/2018/4315861 |
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