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Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV iso...

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Autores principales: Wu, Xiangju, Qi, Jing, Cong, Xiaoyan, Chen, Lei, Hu, Yue, Yoo, Dongwan, Wang, Guisheng, Tian, Fulin, Li, Feng, Sun, Wenbo, Chen, Zhi, Guo, Lihui, Wu, Jiaqiang, Li, Jun, Wang, Jinbao, Zhao, Xiaomin, Du, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841097/
https://www.ncbi.nlm.nih.gov/pubmed/29682543
http://dx.doi.org/10.1155/2018/4315861
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author Wu, Xiangju
Qi, Jing
Cong, Xiaoyan
Chen, Lei
Hu, Yue
Yoo, Dongwan
Wang, Guisheng
Tian, Fulin
Li, Feng
Sun, Wenbo
Chen, Zhi
Guo, Lihui
Wu, Jiaqiang
Li, Jun
Wang, Jinbao
Zhao, Xiaomin
Du, Yijun
author_facet Wu, Xiangju
Qi, Jing
Cong, Xiaoyan
Chen, Lei
Hu, Yue
Yoo, Dongwan
Wang, Guisheng
Tian, Fulin
Li, Feng
Sun, Wenbo
Chen, Zhi
Guo, Lihui
Wu, Jiaqiang
Li, Jun
Wang, Jinbao
Zhao, Xiaomin
Du, Yijun
author_sort Wu, Xiangju
collection PubMed
description Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.
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spelling pubmed-58410972018-04-21 Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line Wu, Xiangju Qi, Jing Cong, Xiaoyan Chen, Lei Hu, Yue Yoo, Dongwan Wang, Guisheng Tian, Fulin Li, Feng Sun, Wenbo Chen, Zhi Guo, Lihui Wu, Jiaqiang Li, Jun Wang, Jinbao Zhao, Xiaomin Du, Yijun Biomed Res Int Research Article Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV. Hindawi 2018-02-21 /pmc/articles/PMC5841097/ /pubmed/29682543 http://dx.doi.org/10.1155/2018/4315861 Text en Copyright © 2018 Xiangju Wu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Xiangju
Qi, Jing
Cong, Xiaoyan
Chen, Lei
Hu, Yue
Yoo, Dongwan
Wang, Guisheng
Tian, Fulin
Li, Feng
Sun, Wenbo
Chen, Zhi
Guo, Lihui
Wu, Jiaqiang
Li, Jun
Wang, Jinbao
Zhao, Xiaomin
Du, Yijun
Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title_full Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title_fullStr Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title_full_unstemmed Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title_short Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line
title_sort establishment and characterization of a high and stable porcine cd163-expressing marc-145 cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841097/
https://www.ncbi.nlm.nih.gov/pubmed/29682543
http://dx.doi.org/10.1155/2018/4315861
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