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Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors
Recent canine influenza outbreaks have raised concerns about the generation of pathogenic variants that may pose a threat to public health. Here, we examine avian-like H3N2 canine influenza viruses (CIVs) isolated from 2009 to 2013 in South Korea from dogs. Phylogenetic analysis revealed that these...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841232/ https://www.ncbi.nlm.nih.gov/pubmed/29511200 http://dx.doi.org/10.1038/s41426-017-0013-x |
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author | Lee, In-Won Kim, Young-Il Lim, Gyo-Jin Kwon, Hyeok-Il Si, Young-Jae Park, Su-Jin Kim, Eun-Ha Kim, Se Mi Nguyen, Hiep Dinh Song, Min-Suk Choi, Young-Ki |
author_facet | Lee, In-Won Kim, Young-Il Lim, Gyo-Jin Kwon, Hyeok-Il Si, Young-Jae Park, Su-Jin Kim, Eun-Ha Kim, Se Mi Nguyen, Hiep Dinh Song, Min-Suk Choi, Young-Ki |
author_sort | Lee, In-Won |
collection | PubMed |
description | Recent canine influenza outbreaks have raised concerns about the generation of pathogenic variants that may pose a threat to public health. Here, we examine avian-like H3N2 canine influenza viruses (CIVs) isolated from 2009 to 2013 in South Korea from dogs. Phylogenetic analysis revealed that these viruses are closely related to strains previously isolated from dogs in Korea and China. However, molecular characterization demonstrated non-synonymous mutations between the canine viruses, particularly in the putative H3 antigenic sites, NA stalk regions, and in the internal genes of the 2012–2013 isolates compared with the 2009 isolate. Animal experiments showed that three representative isolates (A/canine/Korea/AS-01/2009(AS-01/09), A/canine/Korea/AS-05/2012(AS-05/12) and A/canine/Korea/AS-11/2013(AS-11/13), were readily droplet transmitted between dogs, whereas AS-05/12 induced more severe clinical disease and was lethal in dogs compared with AS-01/09. Although all viruses were able to infect ferrets, AS-05/12 consistently yielded higher nasal wash titers and was transmissible to ferrets via airborne droplets. Using reverse genetics, we show that the NA, NP, and M genes of CIV are critical for the adaptation of avian H3N2 viruses, and the resulting reassortant genotypes promote viral growth in dogs in a manner similar to that of the wild-type AS-01/09 virus. Taken together, these results demonstrate that CIVs continuously evolve in dogs thereby allowing them to gain a foothold in mammalian hosts. Importantly, we elucidated the genetic contributions of the NA, NP, and M genes to the adaptability of CIVs derived from the avian H3N2 virus. |
format | Online Article Text |
id | pubmed-5841232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58412322018-03-08 Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors Lee, In-Won Kim, Young-Il Lim, Gyo-Jin Kwon, Hyeok-Il Si, Young-Jae Park, Su-Jin Kim, Eun-Ha Kim, Se Mi Nguyen, Hiep Dinh Song, Min-Suk Choi, Young-Ki Emerg Microbes Infect Article Recent canine influenza outbreaks have raised concerns about the generation of pathogenic variants that may pose a threat to public health. Here, we examine avian-like H3N2 canine influenza viruses (CIVs) isolated from 2009 to 2013 in South Korea from dogs. Phylogenetic analysis revealed that these viruses are closely related to strains previously isolated from dogs in Korea and China. However, molecular characterization demonstrated non-synonymous mutations between the canine viruses, particularly in the putative H3 antigenic sites, NA stalk regions, and in the internal genes of the 2012–2013 isolates compared with the 2009 isolate. Animal experiments showed that three representative isolates (A/canine/Korea/AS-01/2009(AS-01/09), A/canine/Korea/AS-05/2012(AS-05/12) and A/canine/Korea/AS-11/2013(AS-11/13), were readily droplet transmitted between dogs, whereas AS-05/12 induced more severe clinical disease and was lethal in dogs compared with AS-01/09. Although all viruses were able to infect ferrets, AS-05/12 consistently yielded higher nasal wash titers and was transmissible to ferrets via airborne droplets. Using reverse genetics, we show that the NA, NP, and M genes of CIV are critical for the adaptation of avian H3N2 viruses, and the resulting reassortant genotypes promote viral growth in dogs in a manner similar to that of the wild-type AS-01/09 virus. Taken together, these results demonstrate that CIVs continuously evolve in dogs thereby allowing them to gain a foothold in mammalian hosts. Importantly, we elucidated the genetic contributions of the NA, NP, and M genes to the adaptability of CIVs derived from the avian H3N2 virus. Nature Publishing Group UK 2018-03-07 /pmc/articles/PMC5841232/ /pubmed/29511200 http://dx.doi.org/10.1038/s41426-017-0013-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, In-Won Kim, Young-Il Lim, Gyo-Jin Kwon, Hyeok-Il Si, Young-Jae Park, Su-Jin Kim, Eun-Ha Kim, Se Mi Nguyen, Hiep Dinh Song, Min-Suk Choi, Young-Ki Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title | Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title_full | Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title_fullStr | Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title_full_unstemmed | Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title_short | Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors |
title_sort | comparison of the virulence and transmissibility of canine h3n2 influenza viruses and characterization of their canine adaptation factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841232/ https://www.ncbi.nlm.nih.gov/pubmed/29511200 http://dx.doi.org/10.1038/s41426-017-0013-x |
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