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A combined method for DNA analysis and radiocarbon dating from a single sample

Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both a...

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Autores principales: Korlević, Petra, Talamo, Sahra, Meyer, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841407/
https://www.ncbi.nlm.nih.gov/pubmed/29515143
http://dx.doi.org/10.1038/s41598-018-22472-w
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author Korlević, Petra
Talamo, Sahra
Meyer, Matthias
author_facet Korlević, Petra
Talamo, Sahra
Meyer, Matthias
author_sort Korlević, Petra
collection PubMed
description Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both ancient DNA sequences and radiocarbon dates from the same sample material. This is achieved by releasing DNA from the bone matrix through incubation with either EDTA or phosphate buffer prior to complete demineralization and collagen extraction utilizing the acid-base-acid-gelatinization and ultrafiltration procedure established in most radiocarbon dating laboratories. Using a set of 12 bones of different ages and preservation conditions we demonstrate that on average 89% of the DNA can be released from sample powder with minimal, or 38% without any, detectable collagen loss. We also detect no skews in radiocarbon dates compared to untreated samples. Given the different material demands for radiocarbon dating (500 mg of bone/dentine) and DNA analysis (10–100 mg), combined DNA and collagen extraction not only streamlines the sampling process but also drastically increases the amount of DNA that can be recovered from limited sample material.
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spelling pubmed-58414072018-03-14 A combined method for DNA analysis and radiocarbon dating from a single sample Korlević, Petra Talamo, Sahra Meyer, Matthias Sci Rep Article Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both ancient DNA sequences and radiocarbon dates from the same sample material. This is achieved by releasing DNA from the bone matrix through incubation with either EDTA or phosphate buffer prior to complete demineralization and collagen extraction utilizing the acid-base-acid-gelatinization and ultrafiltration procedure established in most radiocarbon dating laboratories. Using a set of 12 bones of different ages and preservation conditions we demonstrate that on average 89% of the DNA can be released from sample powder with minimal, or 38% without any, detectable collagen loss. We also detect no skews in radiocarbon dates compared to untreated samples. Given the different material demands for radiocarbon dating (500 mg of bone/dentine) and DNA analysis (10–100 mg), combined DNA and collagen extraction not only streamlines the sampling process but also drastically increases the amount of DNA that can be recovered from limited sample material. Nature Publishing Group UK 2018-03-07 /pmc/articles/PMC5841407/ /pubmed/29515143 http://dx.doi.org/10.1038/s41598-018-22472-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Korlević, Petra
Talamo, Sahra
Meyer, Matthias
A combined method for DNA analysis and radiocarbon dating from a single sample
title A combined method for DNA analysis and radiocarbon dating from a single sample
title_full A combined method for DNA analysis and radiocarbon dating from a single sample
title_fullStr A combined method for DNA analysis and radiocarbon dating from a single sample
title_full_unstemmed A combined method for DNA analysis and radiocarbon dating from a single sample
title_short A combined method for DNA analysis and radiocarbon dating from a single sample
title_sort combined method for dna analysis and radiocarbon dating from a single sample
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841407/
https://www.ncbi.nlm.nih.gov/pubmed/29515143
http://dx.doi.org/10.1038/s41598-018-22472-w
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