Cargando…

Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex

Four slowly growing mycobacteria isolates were isolated from the respiratory tract and soft tissue biopsies collected in four unrelated patients in Iran. Conventional phenotypic tests indicated that these four isolates were identical to Mycobacterium lentiflavum while 16S rRNA gene sequencing yielde...

Descripción completa

Detalles Bibliográficos
Autores principales: Bouam, Amar, Heidarieh, Parvin, Shahraki, Abodolrazagh Hashemi, Pourahmad, Fazel, Mirsaeidi, Mehdi, Hashemzadeh, Mohamad, Baptiste, Emeline, Armstrong, Nicholas, Levasseur, Anthony, Robert, Catherine, Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841666/
https://www.ncbi.nlm.nih.gov/pubmed/29515197
http://dx.doi.org/10.1038/s41598-018-22526-z
_version_ 1783304781795688448
author Bouam, Amar
Heidarieh, Parvin
Shahraki, Abodolrazagh Hashemi
Pourahmad, Fazel
Mirsaeidi, Mehdi
Hashemzadeh, Mohamad
Baptiste, Emeline
Armstrong, Nicholas
Levasseur, Anthony
Robert, Catherine
Drancourt, Michel
author_facet Bouam, Amar
Heidarieh, Parvin
Shahraki, Abodolrazagh Hashemi
Pourahmad, Fazel
Mirsaeidi, Mehdi
Hashemzadeh, Mohamad
Baptiste, Emeline
Armstrong, Nicholas
Levasseur, Anthony
Robert, Catherine
Drancourt, Michel
author_sort Bouam, Amar
collection PubMed
description Four slowly growing mycobacteria isolates were isolated from the respiratory tract and soft tissue biopsies collected in four unrelated patients in Iran. Conventional phenotypic tests indicated that these four isolates were identical to Mycobacterium lentiflavum while 16S rRNA gene sequencing yielded a unique sequence separated from that of M. lentiflavum. One representative strain AFP-003(T) was characterized as comprising a 6,121,237-bp chromosome (66.24% guanosine-cytosine content) encoding for 5,758 protein-coding genes, 50 tRNA and one complete rRNA operon. A total of 2,876 proteins were found to be associated with the mobilome, including 195 phage proteins. A total of 1,235 proteins were found to be associated with virulence and 96 with toxin/antitoxin systems. The genome of AFP-003(T) has the genetic potential to produce secondary metabolites, with 39 genes found to be associated with polyketide synthases and non-ribosomal peptide syntases and 11 genes encoding for bacteriocins. Two regions encoding putative prophages and three OriC regions separated by the dnaA gene were predicted. Strain AFP-003(T) genome exhibits 86% average nucleotide identity with Mycobacterium genavense genome. Genetic and genomic data indicate that strain AFP-003(T) is representative of a novel Mycobacterium species that we named Mycobacterium ahvazicum, the nineteenth species of the expanding Mycobacterium simiae complex.
format Online
Article
Text
id pubmed-5841666
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-58416662018-03-14 Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex Bouam, Amar Heidarieh, Parvin Shahraki, Abodolrazagh Hashemi Pourahmad, Fazel Mirsaeidi, Mehdi Hashemzadeh, Mohamad Baptiste, Emeline Armstrong, Nicholas Levasseur, Anthony Robert, Catherine Drancourt, Michel Sci Rep Article Four slowly growing mycobacteria isolates were isolated from the respiratory tract and soft tissue biopsies collected in four unrelated patients in Iran. Conventional phenotypic tests indicated that these four isolates were identical to Mycobacterium lentiflavum while 16S rRNA gene sequencing yielded a unique sequence separated from that of M. lentiflavum. One representative strain AFP-003(T) was characterized as comprising a 6,121,237-bp chromosome (66.24% guanosine-cytosine content) encoding for 5,758 protein-coding genes, 50 tRNA and one complete rRNA operon. A total of 2,876 proteins were found to be associated with the mobilome, including 195 phage proteins. A total of 1,235 proteins were found to be associated with virulence and 96 with toxin/antitoxin systems. The genome of AFP-003(T) has the genetic potential to produce secondary metabolites, with 39 genes found to be associated with polyketide synthases and non-ribosomal peptide syntases and 11 genes encoding for bacteriocins. Two regions encoding putative prophages and three OriC regions separated by the dnaA gene were predicted. Strain AFP-003(T) genome exhibits 86% average nucleotide identity with Mycobacterium genavense genome. Genetic and genomic data indicate that strain AFP-003(T) is representative of a novel Mycobacterium species that we named Mycobacterium ahvazicum, the nineteenth species of the expanding Mycobacterium simiae complex. Nature Publishing Group UK 2018-03-07 /pmc/articles/PMC5841666/ /pubmed/29515197 http://dx.doi.org/10.1038/s41598-018-22526-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bouam, Amar
Heidarieh, Parvin
Shahraki, Abodolrazagh Hashemi
Pourahmad, Fazel
Mirsaeidi, Mehdi
Hashemzadeh, Mohamad
Baptiste, Emeline
Armstrong, Nicholas
Levasseur, Anthony
Robert, Catherine
Drancourt, Michel
Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title_full Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title_fullStr Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title_full_unstemmed Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title_short Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex
title_sort mycobacterium ahvazicum sp. nov., the nineteenth species of the mycobacterium simiae complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841666/
https://www.ncbi.nlm.nih.gov/pubmed/29515197
http://dx.doi.org/10.1038/s41598-018-22526-z
work_keys_str_mv AT bouamamar mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT heidariehparvin mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT shahrakiabodolrazaghhashemi mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT pourahmadfazel mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT mirsaeidimehdi mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT hashemzadehmohamad mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT baptisteemeline mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT armstrongnicholas mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT levasseuranthony mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT robertcatherine mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex
AT drancourtmichel mycobacteriumahvazicumspnovthenineteenthspeciesofthemycobacteriumsimiaecomplex