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Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

12–14 days of culturing of bone marrow (BM) cells containing various growth factors is widely used method for generating dendritic cells (DCs) from suspended cell population. Here we compared flask culture method and commercially available CD11c Positive Selection kit method. Immature BMDCs' pu...

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Detalles Bibliográficos
Autores principales: Gosavi, Rahul Ashok, Salwe, Sukeshani, Mukherjee, Sandeepan, Dahake, Ritwik, Kothari, Sweta, Patel, Vainav, Chowdhary, Abhay, Deshmukh, Ranjana A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842714/
https://www.ncbi.nlm.nih.gov/pubmed/29682352
http://dx.doi.org/10.1155/2018/3495086