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Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages

Increased consumption of Western-type diets and environmental insults lead to wide-spread increases in the plasma levels of saturated fatty acids and lipoprotein oxidation. The aim of this study is to examine whether palmitate and minimally modified low-density lipoprotein (mmLDL) exert an additive...

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Autores principales: Ann, Soo-jin, Kim, Ka-Kyung, Cheon, Eun Jeong, Noh, Hye-Min, Hwang, Inhwa, Yu, Je-Wook, Park, Sungha, Kang, Seok-Min, Manabe, Ichiro, Miller, Yury I., Kim, Sangwoo, Lee, Sang-Hak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843266/
https://www.ncbi.nlm.nih.gov/pubmed/29518116
http://dx.doi.org/10.1371/journal.pone.0193649
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author Ann, Soo-jin
Kim, Ka-Kyung
Cheon, Eun Jeong
Noh, Hye-Min
Hwang, Inhwa
Yu, Je-Wook
Park, Sungha
Kang, Seok-Min
Manabe, Ichiro
Miller, Yury I.
Kim, Sangwoo
Lee, Sang-Hak
author_facet Ann, Soo-jin
Kim, Ka-Kyung
Cheon, Eun Jeong
Noh, Hye-Min
Hwang, Inhwa
Yu, Je-Wook
Park, Sungha
Kang, Seok-Min
Manabe, Ichiro
Miller, Yury I.
Kim, Sangwoo
Lee, Sang-Hak
author_sort Ann, Soo-jin
collection PubMed
description Increased consumption of Western-type diets and environmental insults lead to wide-spread increases in the plasma levels of saturated fatty acids and lipoprotein oxidation. The aim of this study is to examine whether palmitate and minimally modified low-density lipoprotein (mmLDL) exert an additive effect on macrophage activation. We found that CXCL2 and TNF-α secretion as well as ERK and p38 phosphorylation were additively increased by co-treatment of J774 macrophages with palmitate and mmLDL in the presence of lipopolysaccharide (LPS). Furthermore, the analysis of differentially expressed genes using the KEGG database revealed that several pathways, including cytokine-cytokine receptor interaction, and genes were significantly altered. These results were validated with real-time PCR, showing upregulation of Il-6, Csf3, Il-1β, and Clec4d. The present study demonstrated that palmitate and mmLDL additively potentiate the LPS-induced activation of macrophages. These results suggest the existence of synergistic mechanisms by which saturated fatty acids and oxidized lipoproteins activate immune cells.
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spelling pubmed-58432662018-03-23 Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages Ann, Soo-jin Kim, Ka-Kyung Cheon, Eun Jeong Noh, Hye-Min Hwang, Inhwa Yu, Je-Wook Park, Sungha Kang, Seok-Min Manabe, Ichiro Miller, Yury I. Kim, Sangwoo Lee, Sang-Hak PLoS One Research Article Increased consumption of Western-type diets and environmental insults lead to wide-spread increases in the plasma levels of saturated fatty acids and lipoprotein oxidation. The aim of this study is to examine whether palmitate and minimally modified low-density lipoprotein (mmLDL) exert an additive effect on macrophage activation. We found that CXCL2 and TNF-α secretion as well as ERK and p38 phosphorylation were additively increased by co-treatment of J774 macrophages with palmitate and mmLDL in the presence of lipopolysaccharide (LPS). Furthermore, the analysis of differentially expressed genes using the KEGG database revealed that several pathways, including cytokine-cytokine receptor interaction, and genes were significantly altered. These results were validated with real-time PCR, showing upregulation of Il-6, Csf3, Il-1β, and Clec4d. The present study demonstrated that palmitate and mmLDL additively potentiate the LPS-induced activation of macrophages. These results suggest the existence of synergistic mechanisms by which saturated fatty acids and oxidized lipoproteins activate immune cells. Public Library of Science 2018-03-08 /pmc/articles/PMC5843266/ /pubmed/29518116 http://dx.doi.org/10.1371/journal.pone.0193649 Text en © 2018 Ann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ann, Soo-jin
Kim, Ka-Kyung
Cheon, Eun Jeong
Noh, Hye-Min
Hwang, Inhwa
Yu, Je-Wook
Park, Sungha
Kang, Seok-Min
Manabe, Ichiro
Miller, Yury I.
Kim, Sangwoo
Lee, Sang-Hak
Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title_full Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title_fullStr Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title_full_unstemmed Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title_short Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
title_sort palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843266/
https://www.ncbi.nlm.nih.gov/pubmed/29518116
http://dx.doi.org/10.1371/journal.pone.0193649
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