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Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors

During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-s...

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Autores principales: Streichan, Sebastian J, Lefebvre, Matthew F, Noll, Nicholas, Wieschaus, Eric F, Shraiman, Boris I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843464/
https://www.ncbi.nlm.nih.gov/pubmed/29424685
http://dx.doi.org/10.7554/eLife.27454
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author Streichan, Sebastian J
Lefebvre, Matthew F
Noll, Nicholas
Wieschaus, Eric F
Shraiman, Boris I
author_facet Streichan, Sebastian J
Lefebvre, Matthew F
Noll, Nicholas
Wieschaus, Eric F
Shraiman, Boris I
author_sort Streichan, Sebastian J
collection PubMed
description During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow.
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spelling pubmed-58434642018-03-12 Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors Streichan, Sebastian J Lefebvre, Matthew F Noll, Nicholas Wieschaus, Eric F Shraiman, Boris I eLife Physics of Living Systems During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow. eLife Sciences Publications, Ltd 2018-02-09 /pmc/articles/PMC5843464/ /pubmed/29424685 http://dx.doi.org/10.7554/eLife.27454 Text en © 2018, Streichan et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Streichan, Sebastian J
Lefebvre, Matthew F
Noll, Nicholas
Wieschaus, Eric F
Shraiman, Boris I
Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title_full Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title_fullStr Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title_full_unstemmed Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title_short Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
title_sort global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843464/
https://www.ncbi.nlm.nih.gov/pubmed/29424685
http://dx.doi.org/10.7554/eLife.27454
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