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An improved MS2 system for accurate reporting of the mRNA life cycle

The MS2-MCP system allows imaging multiple steps of the mRNA life cycle with high temporal and spatial resolution. However for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, confounding the study of...

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Detalles Bibliográficos
Autores principales: Tutucci, Evelina, Vera, Maria, Biswas, Jeetayu, Garcia, Jennifer, Parker, Roy, Singer, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843578/
https://www.ncbi.nlm.nih.gov/pubmed/29131164
http://dx.doi.org/10.1038/nmeth.4502